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| Spolidorio,Denise Madalena Palomari; Höfling,José Francisco; Pizzolitto,Antônio Carlos; Rosa,Edvaldo Antonio; Negrini,Thaís de Cássia; Spolidorio,Luís Carlos. |
| The aim of this study was to determine whether random amplified polymorphic DNA (AP-PCR) analysis is able to differentiate genetically different clones of mutans streptococci, in 22 Brazilian family members. Stimulated saliva samples were collected from fathers, mothers and infants. For 5-18 months babies with erupting primary dentition, plaque samples were collected using sterile tooth pick tips. From these samples, mutans streptococci were isolated on SB-20 agar plates. After growth, representative colonies were identified by biochemical methods on the basis of carbohydrate fermentation. Streptococcus mutans isolates were obtained from all family members and AP-PCR typed separately with a random primer (OPA-13). Bacterial cell lysates were used as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus mutans; AP-PCR; Genetic polymorphism. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300006 |
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