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| Scheffer, K. C.; Di Gregorio, M. C.; Batista, A. M.; Rosato, P. N.; Campos Filho, E.; Silva, A. C. R.; Caporale, G. M. M.; Cruz, P. S.; Chaves, L. B.. |
| Among the factors that establish the pre‐analytical variability, some studies report that hemolysis is the main interferential in several tests. However, no studies were found on immunofluorescence tests or that using cell cultures. The aim of this study was to evaluate the interference of different degrees of hemolysis serum samples in the evaluation of virus neutralizing antibodies (VNA) by rapid fluorescent focus inhibition test (RFFIT). We obtained 27 blood samples from dogs vaccinated for rabies. Hemolysis was caused by mechanical induction and freezing. Hemolysated sera were classified visually and by measurement of hemoglobin made by spectrophotometry. The evaluation of VNA in the samples was performed by RFFIT. The results were statistically... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17379 |
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