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Lee,Sarah Hwa In; Barancelli,Giovana Verginia; Corassin,Carlos Humberto; Rosim,Roice Eliana; Coppa,Carolina Fernanda Sengling Cebin; Oliveira,Carlos Augusto Fernandes de. |
ABSTRACT This study aimed to investigate the effect of peracetic acid (PAA, 0.5%) on adherent cells of three strains of Listeria monocytogenes strains belonging to serotypes 4b and 1/2b that had been previously isolated from the environment of a Brazilian cheese plant. The assays were conducted using polystyrene microplates and stainless steel coupons and the adhered cells were treated with PAA for 60, 120 and 180 s. On stainless steel, biofilms were partially inactivated by PAA after 60 s and almost 100% of the cells were damaged within 180 s using epifluorescence microscopy with LIVE/DEAD® staining. On polystyrene microplates, PAA decreased (P<0.05) biofilm biomass produced by the three L. monocytogenes isolates at 60 s, when compared with controls... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peracetic acid (PAA)/ biofilm/effects; L. monocytogenes; Dairy plants/Brazil. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300610 |
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Oliveira,Carlos Augusto Fernandes de; Sebastião,Luciana Soares; Fagundes,Helena; Rosim,Roice Eliana; Fernandes,Andrezza Maria. |
A ocorrência de aflatoxina B1 (AFB1) em rações e aflatoxina M1 (AFM1) no leite cru foi avaliada em propriedades leiteiras situadas na região nordeste do Estado de São Paulo, Brasil, de outubro de 2005 a fevereiro de 2006. A análise de aflatoxinas foi efetuada utilizando-se colunas de imunoafinidade para purificação dos extratos, sendo a quantificação realizada através de cromatografia líquida de alta eficiência. A AFB1 foi detectada em 40% das rações em níveis de 1,0 a 19,5 μg.kg-1. A concentração de AFM1 em 36,7% de amostras de leite positivas variou de 0,010 a 0,645 μg.L-1. Somente uma amostra de leite estava acima do limite de tolerância adotado no Brasil (0,5 μg.L-1) para AFM1. Concluiu-se que as concentrações de aflatoxinas na ração e no leite foram... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micotoxinas; Resíduos; Ocorrência. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000500034 |
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Fernandes,Andrezza Maria; Bovo,Fernanda; Moretti,Thais Santos; Rosim,Roice Eliana; Lima,César Gonçalves de; Oliveira,Carlos Augusto Fernandes de. |
The objective of this work was to evaluate the effect of somatic cell counts (SCC) in casein fractions of ultra high temperature (UHT) milk. Raw milks were categorized in SCC groups of low (200,000-320,000 cells mL-1), intermediate (380,000-560,000 cells mL-1) and high cells (600,000-800,000 cells mL-1). Five replicates of UHT milks within each SCC category were analyzed for casein fractions after 8, 30, 60, 90 and 120 days of storage through high performance liquid chromatography. SCC showed effect only on beta-casein reduction. SCC in raw milk increases the proteolysis of UHT milk, as a consequence of beta-casein degradation. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SCC; UHT milk; Proteolysis; Milk quality. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000100019 |
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