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| Houllou-Kido,Laureen Michelle; Kido,Ederson Akio; Falco,Maria Cristina; Silva Filho,Márcio de Castro; Figueira,Antônio Vargas de Oliveira; Nogueira,Neusa de Lima; Rossi,Mônica Lanzoni; Tulmann Neto,Augusto. |
| A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture, somatic embryos started to regenerate. The maximum number of regenerated plants was observed between 45 and 60 days after embryo formation.In the first experiment,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa; Cell suspension; Callus; Somatic differentiation; Biobalistic. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001100005 |
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| Lavres Junior,José; Malavolta,Eurípedes; Nogueira,Neusa de Lima; Moraes,Milton Ferreira; Reis,André Rodrigues; Rossi,Mônica Lanzoni; Cabral,Cleusa Pereira. |
| The deleterious effects of both Mn deficiency and excess on the development of plants have been evaluated with regard to aspects of shoot anatomy, ultrastructure and biochemistry, focusing mainly on the manifestation of visual symptoms. However, there is little information in the literature on changes in the root system in response to Mn supply. The objective of this study was to evaluate the effects of Mn doses (0.5, 2.0 and 200.0 μmol L-1) in a nutrient solution on the anatomy of leaves and roots of the Glycine max (L.) cultivars Santa Rosa, IAC-15 and IAC-Foscarin 31. Visual deficiency symptoms were first observed in Santa Rosa and IAC-15, which were also the only cultivars where Mn-toxicity symptoms were observed. Only in IAC-15, a high Mn supply led... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoplast; Glycine max; Optical microscopy; Root diameter. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000200017 |
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| Lavres Junior,José; Cabral,Cleusa Pereira; Rossi,Mônica Lanzoni; Nogueira,Thiago Assis Rodrigues; Nogueira,Neusa de Lima; Malavolta,Eurípedes. |
| Castor bean is a nutrient-demanding species, but there is still little information on its micronutrient requirements. The objectives of this study were to evaluate the effects of levels of B (2.5, 12.5 and 25.0 µmol L-1), Cu (0.05, 0.25 and 0.50 µmol L-1), Mn (0.2, 1.0 and 2.0 µmol L-1) and Zn (0.2, 1.0 and 2.0 µmol L-1) in a nutrient solution on plant B, Cu, Mn and Zn concentrations and uptake, vegetative growth and fruit yield of castor bean "Iris", grown in greenhouse. The experiment was arranged in a completely randomized block design with three replicates. The first deficiency symptoms were observed for B, followed by Zn, Cu and Mn. The main changes in the cell ultrastructure due to lack of B were thickening of the cell walls and middle lamellae,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nutritional requirement; Ricinus communis L; Cell ultrastructure. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832012000100024 |
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| Lavres Junior,José; Reis,André Rodrigues; Rossi,Mônica Lanzoni; Cabral,Cleusa Pereira; Nogueira,Neusa de Lima; Malavolta,Eurípedes. |
| The deleterious effects of Mn stress on many species have been studied, mainly concerning biochemical, physiological and growth parameters of plants. However, there are few studies relating the anatomical and ultrastructural changes in response to manganese (Mn) nutritional disorders, This study examined the leaf ultrastructure of Mn-inefficient (IAC-15, Santa Rosa) and Mn-efficient (IAC-Foscarin 31) soybean (Glycine max L.) genotypes in response to three rates of Mn (0.5, 2 and 200 µmol L-1) in the nutrient solution. Symptoms of Mn deficiency developed 12 days after transplanting in IAC-15 and Santa Rosa, followed by IAC-Foscarin 31 on the 15th day. Only IAC-15 and Santa Rosa leaves showed symptoms of Mn toxicity. The Mn concentration in leaves ranged... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chloroplast; Deficiency; Plant nutrition; Toxicity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000300006 |
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| Soares,Taliane Leila; Souza,Everton Hilo de; Rossi,Mônica Lanzoni; Souza,Fernanda Vidigal Duarte. |
| O objetivo do trabalho foi avaliar a viabilidade de grãos de pólen de acessos silvestres de abacaxi mediante a percentagem de germinação in vitro, crescimento do tubo polínico em duas temperaturas de incubação e uso de carmim acético. Além disso, o trabalho busca reconhecer os padrões da morfologia polínica de algumas variedades de abacaxi. Foram utilizadas amostras de pólen coletadas de seis acessos de abacaxi: Silvestre-25, I-26/803 (Ananas macrodontes), Igor (A. comosus var. erectifolius), Ananás Santo Amaro, FRF-22 e FRF-32 (A. comosus var. bracteatus). Os grãos de pólen foram inoculados em meio de cultura contendo 10% de sacarose, 0,01% H3BO3, 0,01% KNO3, 0,03% Ca(NO3)2.4H2O, solidificado com 1% de ágar e pH ajustado para 6,5. Foram testadas as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ananas; Germinação in vitro; Tubo polínico; Carmim acético; Plantas ornamentais; Cultura in vitro. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001000011 |
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| Rodrigues,Fabíola Rebouças; Almeida,Weliton Antonio Bastos de; Ledo,Carlos Alberto da Silva; Soares,Taliane Leila; Rossi,Mônica Lanzoni; Santana,José Raniere Ferreira de. |
| ABSTRACT Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alumã; In vitro culture of calli; Anatomical analysis; Scanning electron microscopy; Growth regulators.. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100201 |
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