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Genetic diversity of human immunodeficiency virus-1 isolates in Paraná, Brazil BJID
Toledo,Paula Virginia Michelon; Carvalho,Denise Siqueira de; Rossi,Silvia Gomes de; Brindeiro,Rodrigo; Queiroz-Telles,Flávio de.
OBJECTIVE: Because epidemiological data on circulating HIV subtypes among HIV-positive patients in the state of Paraná were not known until now, the aims of this study were to describe the genetic diversity profile of HIV-1 in treated patients in Paraná, Brazil, and report the differences in protease (PR) and reverse transcriptase (RT) mutations in HIV-1 subtypes. PATIENTS AND METHODS: A cross-sectional study was conducted from 2003 to 2006. Plasma viral RNA of 389 patients was extracted and PR and RT genes were polymerase chain reaction-amplified and sequenced. Sequences were subtyped and examined for antiretroviral resistance mutations. Data on gender of patient harboring the viruses and past history of antiretroviral treatment were also collected....
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV-1 subtypes; Genotype; Antiretroviral; Drug experienced patients.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300006
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