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| Santos,Ivo Walter dos; Nóbrega Junior,Jandui Escarião da; Cesaro,Matheus Pedrotti de; Ilha,Gustavo Freitas; Rovani,Monique Tomazele; Gonçalves,Paulo Bayard Dias. |
| O presente estudo teve como objetivo avaliar a capacidade crioprotetora das lipoproteínas de baixa densidade (LDL) presentes no plasma de gema de ovo, adicionado ao trihidroxiaminometano (TRIS) para congelar sêmen ovino. Trinta e seis ejaculados foram coletados para formar 12 "pool". Cada alíquota de sêmen foi diluída em TRIS-gema de ovo (TRISG) ou TRIS- plasma de gema de ovo (TRISP) antes de congelar o sêmen. Para a obtenção do plasma da gema de ovo, foi utilizado o método de ultracentrifugação. Após o descongelamento, não houve diferença entre os dois extensores em relação aos parâmetros seminais (motilidade, viabilidade, membrana acrossômica e plasma). No entanto, no Teste de Termo Resistência Lenta (TTRL - 4h/38°C), o sêmen congelado com TRISP resultou... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Espermatozoide; Ovino; Lipoproteína de baixa densidade; TRIS.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601107 |
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| Rovani,Monique Tomazele; Cezar,Alfredo Skrebsky; Rigo,Melânia Lazzari; Gasperin,Bernardo Garziera; Nóbrega Júnior,Janduí Escarião da; Torres,Fabrício Dias; Gonçalves,Paulo Bayard Dias; Ferreira,Rogério. |
| ABSTRACT: Pregnancy diagnosis is an important tool for farm management. Ultrasonography is the main technique used for pregnancy diagnosis in ewes. As an alternative, radioimmunoassay (RIA) allows accurate and early detection of pregnancy-associated glycoproteins (PAGs) in sheep blood. However, radioactive-based techniques, as RIA, have been increasingly inadvisable due to environmental risk. Homology between ovine and bovine PAGs is high, and ELISA kits used for PAGs detection in cattle are safer than RIA. Thus, this study aimed to evaluate the feasibility of PAGs detection for pregnancy diagnosis in sheep serum samples using an ELISA kit produced for cattle. The sensitivity and specificity of the ELISA kit were 93.5% and 98.9%, respectively, whereas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pregnancy-associated glycoproteins; Ewe; Pregnancy blood test; ELISA kit. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200362 |
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| Costa,Vitória Gasperin Guazzelli; Vieira,Arnaldo Diniz; Schneider,Augusto; Rovani,Monique Tomazele; Gonçalves,Paulo Bayard Dias; Gasperin,Bernardo Garziera. |
| ABSTRACT: Previous studies have evaluated the effects of different reproductive procedures on discomfort markers in sheep and cattle. Such studies may help stimulate the adoption of techniques that are more beneficial for animal welfare. However, markers that are commonly used to evaluate discomfort are highly influenced by external factors. To overcome this, several systemic markers can be evaluated to more precisely identify stress, pain, and inflammation. Such markers include cortisol, acute phase proteins, bradykinin, and substance P. We aimed to review the potential markers of stress, pain, and inflammation, and discuss how and when they are regulated after different stimuli related to reproductive procedures in cattle and sheep. Furthermore, we... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute phase proteins; Cortisol; Discomfort; Reproductive techniques. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200651 |
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| Haas,Cristina Sangoi; Santos,Monike Quirino dos; Rovani,Monique Tomazele; Santos,Joabel Tonellotto dos; Mellagi,Ana Paula Gonçalves; Bortolozzo,Fernando Pandolfo; Gasperin,Bernardo Garziera; Gonçalves,Paulo Bayard Dias. |
| ABSTRACT: Gestation length in swine has a considerable amplitude and both early and delayed parturition are common. This variation increases the occurrence of unassisted farrowing and could lead to a wide-ranging age at weaning for piglets born from one batch. Supervision of sow parturition is crucial to reduce mortality of neonate piglets. To facilitate assistance, induction of farrowing using prostaglandin F2α (PGF) has been widely used in batch farrowing systems, whereby synchronization would concentrate the time of farrowing, allowing for better organization of employees. However, a viable alternative method that can be implemented to manage farrowing is to sustain high progestagen levels in the final days of gestation and, consequently, prevent early... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Farrowing induction; Intravaginal device; Progestagen; Early parturiton. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100652 |
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