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	Provedor de dados BJMBR (2) Autor Rozental,R. (2) Campos-de-Carvalho,A.C. (1) Spray,D.C (1) Spray,D.C. (1) Urban,M. (1) Palavra-chave Gap junctions (2) CDNA sequences (1) Cardiovascular system (1) Connexins (1) Immune system (1) Intercellular communication (1) Multi primers (1) Universal primers (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2000 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A simple RT-PCR-based strategy for screening connexin identity BJMBR Urban,M.; Rozental,R.; Spray,D.C. Vertebrate gap junctions are aggregates of transmembrane channels which are composed of connexin (Cx) proteins encoded by at least fourteen distinct genes in mammals. Since the same Cx type can be expressed in different tissues and more than one Cx type can be expressed by the same cell, the thorough identification of which connexin is in which cell type and how connexin expression changes after experimental manipulation has become quite laborious. Here we describe an efficient, rapid and simple method by which connexin type(s) can be identified in mammalian tissue and cultured cells using endonuclease cleavage of RT-PCR products generated from "multi primers" (sense primer, degenerate oligonucleotide corresponding to a region of the first extracellular... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gap junctions; Intercellular communication; CDNA sequences; Universal primers; Multi primers. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800014 Gap junctions in the cardiovascular and immune systems BJMBR Rozental,R.; Campos-de-Carvalho,A.C.; Spray,D.C.. Gap junctions are clusters of intercellular channels directly connecting the cytoplasm of adjacent cells. These channels are formed by proteins named connexins and are present in all metazoan organisms where they serve diverse functions ranging from control of cell growth and differentiation to electric conduction in excitable tissues. In this overview we describe the presence of connexins in the cardiovascular and lympho-hematopoietic systems giving the reader a summary of the topics to be covered throughout this edition and a historical perspective of the discovery of gap junctions in the immune system. Tipo: Info:eu-repo/semantics/article Palavras-chave: Gap junctions; Cardiovascular system; Immune system; Connexins. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400001 Registros recuperados: 2 Primeira ... 1 ... Última

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