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| KLOSTERHOFF,MARTA C.; PEREIRA JÚNIOR,JOABER; RODRIGUES,RICARDO V.; GUSMÃO,EMELINE P.; SAMPAIO,LUÍS A.; TESSER,MARCELO B.; ROMANO,LUIS A.. |
| ABSTRACT In the present study was evaluated the ontogenic of immunocompetent organs of cobia up to 53 days after hatching (dah) through histology and immunohistochemistry techniques. The kidney was the first lymphohematopoietic organ to appear, at 1 dah, followed by the spleen at 5 dah and the thymus at 7 dah. The first CD3 receptors on the lymphocytes were observed in 27% of the thymic tissue at 7 dah and in 99% at 53 dah. The phenotypic expression of CD3 receptors was registered in 10% of the kidney at 8 dah and in 32% at 53 dah. CD4 receptors were observed in 5% and 63% of the thymic area at 7 and 53 dah, respectively. In the kidney, T4 lymphocytes were first observed at 13 dah in 9% of the organ and in 28% at 53 dah, defining the functional development... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cobia; Immune system; Ontogeny; Immunohistochemistry; Lymphoid organs. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502111 |
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| OTERO,ADRIANA P.S.; RODRIGUES,RICARDO V.; SAMPAIO,LUÍS A.; ROMANO,LUIS A.; TESSER,MARCELO B.. |
| The aim of this study was to describe the ontogeny of thyroid follicles in cobia Rachycentron canadum. Larvae were sampled daily (n=15 - 20) from hatching until 15 dah (days after hatching). Following, larvae were sampled every two days by 28 dah; a new sample was taken at 53 dah. The samples were dehydrated, embedded in Paraplast, and sections of 3 µm were dewaxed, rehydrated and stained with HE and PAS. A single follicle was already present 1 dah and three follicles were found 8 dah. The number of follicles increased up to 19 on 53 dah. The diameter of follicles and follicular cell height were lower 1 dah (6.83 ± 1.00 and 4.6 ± 0.01 µm), but increased from 8 dah (24.03 ± 0.46 µm e 6.43 ± 0.46 µm). From 8 dah, the presence of reabsorption vesicles was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colloid; Follicles; Larvae; Metamorphosis; Thyroid tissue. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301507 |
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