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	Provedor de dados Braz. J. Vet. Res. Anim. Sci. (2) Autor SANTOS,Silvia Edelweiss Crusco dos (2) VANNUCCHI,Camila Infantosi (2) VISINTIN,José Antonio (2) ASSUMPÇÃO,Mayra Elena Ortiz D’Avila (1) SATZINGER,Sandra (1) Palavra-chave Freezing (2) Semen (2) Cryoprotectants (1) Diluents (1) Dog (1) Dogs (1) Ethylene glycol (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 1999 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última In vitro viability of canine spermatozoa frozen in tris-fructose-citric acid extender with ethylene glycol Braz. J. Vet. Res. Anim. Sci. VANNUCCHI,Camila Infantosi; SANTOS,Silvia Edelweiss Crusco dos; VISINTIN,José Antonio. The use of frozen semen has become increasingly popular in canine artificial insemination, stimulating research to improve freezing methods and extenders. In this study, was compared the final concentration of 5% ethylene glycol used in 3 equilibration protocols. In Method I, one part of semen was added to two parts of tris-fructose-citric acid with 7.5% ethylene glycol (Extender 2) and frozen without cooling. In Method II, one part of semen was added to two parts of tris-fructose-citric acid with 7.5% ethylene glycol (Extender 2) and cooled at 5ºC for 1 hour before freezing. In Method III, one part of semen was added to one part of tris-fructose-citric without ethylene glycol (Extender 1) and cooled at 5ºC for 1 hour. Just after this period, one part of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethylene glycol; Cryoprotectants; Freezing; Semen; Dog. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000400007 Comparison of five extenders for canine semen freezing Braz. J. Vet. Res. Anim. Sci. SANTOS,Silvia Edelweiss Crusco dos; VANNUCCHI,Camila Infantosi; SATZINGER,Sandra; ASSUMPÇÃO,Mayra Elena Ortiz D’Avila; VISINTIN,José Antonio. Semen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diluents; Freezing; Semen; Dogs. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000500004 Registros recuperados: 2 Primeira ... 1 ... Última

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