|
|
|
|
|
| GUIMARAES, P. M.; GUIMARAES, L. A.; MORGANTE, C. V.; SILVA JUNIOR, O. B.; ARAUJO, A. C. G.; MARTINS, A. C. Q.; SARAIVA, M. A. P.; OLIVEIRA, T. N.; TOGAWA, R. C.; LEAL-BERTIOLI, S. C. M.; BERTIOLI, D. J.; BRASILEIRO, A. C. M.. |
| Wild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diver-sity for resistance to biotic and abiotic stresses. The wild diploid A.stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (RKN)Meloidogyne arenaria through the onset of the Hypersensitive Response (HR). In order to identify genes and regulators triggering this defense response, a comprehensive root tran-scriptome analysis during the first stages of this incompatible interaction was conducted using Illumina Hi-Seq. Overall, eight cDNA libraries were produced generating 28.2 GB, which were de novo assembled into 44,132... |
| Tipo: Artigo de periódico |
Palavras-chave: Diplóide selvagem; Nematóide das galhas; Gene regulador; Planta resistente; Melhoramento de planta; Peanut; Arachis spp; Peanut relatives.; Arachis Hypogaea.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035460 |
| |
|
|
| GUIMARAES, P. M.; GUIMARAES, L. A.; MORGANTE, C. V.; SILVA JUNIOR, O. B.; ARAUJO, A. C. G.; MARTINS, A. C. Q.; SARAIVA, M. A. P.; OLIVEIRA, T. N.; TOGAWA, R. C.; LEAL-BERTIOLI, S. C. M.; BERTIOLI, D. J.; BRASILEIRO, A. C. M.. |
| Tipo: Separatas |
Palavras-chave: Peanut.; Arachis Hypogaea.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1030792 |
| |
|
|
| PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M.. |
| Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The... |
| Tipo: Artigo de periódico |
Palavras-chave: Transgenic soybean; Agrobacterium rhizogenes; Glycine Max; Soja; Melhoramento Genético Vegetal; Meloidogyne spp; Soybeans. |
| Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154066 |
| |
|
|
| BRASILEIRO, A. C. M.; MORGANTE, C. V.; ARAUJO, A. C. G.; LEAL-BERTIOLI, S. C. M.; SILVA, A. K.; MARTINS, A. C. Q.; VINSON, C. C.; SANTOS, C. M. R.; BONFIM, O.; TOGAWA, R. C.; SARAIVA, M. A. P.; BERTIOLI, D. J.; GUIMARAES, P. M.. |
| Peanut (Arachis hypogaeaL.) is an important le-gume cultivated mostly in drought-prone areas where its pro-ductivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for pea-nut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysi from leaves and roots of A. duranensis... |
| Tipo: Artigo de periódico |
Palavras-chave: Gene tolerante; Cultura resistente a seca; 454 Sequencing; Differential gene expression; Dry-down; Peanut wild relatives; QRT-PCR; SSHlibraries.; Biologia molecular; Amendoim; Resistência a Seca.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035448 |
| |
|
| |
|
|
|
