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SOUZA ROCHA,Katarine de; MONTEIRO,Louysse Helene; SANTOS MIRANDA,Juliana Maria; MONTEIRO BAIA,Ianny Watuzy; MARQUES MONTEIRO,Thamillys Rayssa; SCHUPP DE SENA MESQUITA,Gleiciane; ROCHA ALBUQUERQUE,Mirian da; THIEMY YOKOKURA,Layna; CASTANHEIRA PIMENTA,Gabriela; TEIXEIRA GOMES,Maria Érika; MARQUES BARROZO,Pedro Henrique; CARVALHO DE CASTRO,Danniel; ABENSUR VIEIRA,André Luiz; EGUCHI MESQUITA,Ellen Yasmin; GUIMARÃES DE MORAES,Carla Cristina. |
ABSTRACT Leptospirosis is a zoonosis transmitted by contact with infected urine or water contaminated with the agent. Searches for Leptospira spp. in reptiles are scarce although most species have contact with aquatic environments. We evaluated the presence of anti-Leptospira spp. antibodies in Podocnemis expansa housed at the Amazonian Zoobotanical Garden, in Belém, Pará state, Brazil. We analyzed 74 serum samples through the microscopic agglutination test using 31 live antigens from different Leptospira spp. serogroups. Thirty samples (40.5%) were positive against Leptospira spp., with titrations between 100 and 3,200 for one or more serogroups. The Hebdomadis serogroup was the most prevalent, with 26 (87%) out of the 30 positive samples, followed by... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Chelonians; Microscopic agglutination test; Hebdomadis; Djasiman; Celledoni; Bataviae. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000200115 |
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SOUZA ROCHA,Katarine de; SCHUPP DE SENA MESQUITA,Gleiciane; SILVA FERREIRA,Maeli Fernanda; LEITE BARROS,Flávia de Nazaré; MACEDO,Renata Cecília Soares de Lima; ARAÚJO SARAIVA,Elane de; MENDES-OLIVEIRA,Ana Cristina; DUARTE CERQUEIRA,Valíria; SCOFIELD,Alessandra; GOES CAVALCANTE,Gustavo; ABEL,Isis; GUIMARÃES DE MORAES,Carla Cristina. |
ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon. |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Didelphimorphia; Rodentia; Live traps; Kidney; Liver; 16S rRNA. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305 |
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