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| SILVA,VICTOR D.A.; CUEVAS,CARLOS; MUÑOZ,PATRICIA; VILLA,MONICA; AHUMADA-CASTRO,ULISES; HUENCHUGUALA,SANDRO; SANTOS,CLEONICE C. DOS; ARAUJO,FILLIPE M. DE; FERREIRA,RAFAEL S; SILVA,VANESSA B. DA; SILVA,JULIANA H.C. E; SOARES,ÉRICA N.; VELOZO,EUDES S.; SEGURA-AGUILAR,JUAN; COSTA,SILVIA L.. |
| ABSTRACT Prosopis juliflora is a shrub that has been used to feed animals and humans. However, a synergistic action of piperidine alkaloids has been suggested to be responsible for neurotoxic damage observed in animals. We investigated the involvement of programmed cell death (PCD) and autophagy on the mechanism of cell death induced by a total extract (TAE) of alkaloids and fraction (F32) from P. juliflora leaves composed majoritary of juliprosopine in a model of neuron/glial cell co-culture. We saw that TAE (30 µg/mL) and F32 (7.5 µg/mL) induced reduction in ATP levels and changes in mitochondrial membrane potential at 12 h exposure. Moreover, TAE and F32 induced caspase-9 activation, nuclear condensation and neuronal death at 16 h exposure. After 4 h,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Programmed cell death; Autophagy; Glial cells; Neurons; Piperidine alkaloids; Prosopis juliflora.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100247 |
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