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	Provedor de dados BJMBR (2) Autor Diaz,R.S. (2) Sabino,E.C. (2) Accetturi,C.A. (1) Caseiro,M. (1) Rossini,M.A.A. (1) Turcato,G. (1) Mais... Palavra-chave Brazil (1) HIV-1 subtypes (1) Heteroduplex mobility analysis (1) Intravenous drug users (1) Molecular epidemiology (1) Polymerase chain reaction fidelity (1) Thermotoga maritima DNA polymerase (1) Thermus aquaticus (Taq) DNA polymerase (1) Mais... Tipo do documento Info:eu-repo/semantics/other (2) Ano 2001 (1) 1998 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil BJMBR Rossini,M.A.A.; Diaz,R.S.; Caseiro,M.; Turcato,G.; Accetturi,C.A.; Sabino,E.C.. In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%)... Tipo: Info:eu-repo/semantics/other Palavras-chave: HIV-1 subtypes; Intravenous drug users; Molecular epidemiology; Heteroduplex mobility analysis; Brazil. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100005 Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase BJMBR Diaz,R.S.; Sabino,E.C.. For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that would survive the high temperatures needed for DNA denaturation. The development of enzymes with an inherent 3' to 5' exonuclease proofreading activity, lacking in Taq polymerase, would be an improvement when higher fidelity is needed. We used the forward mutation assay to compare the fidelity of Taq polymerase and Thermotoga maritima (ULTMA™) DNA polymerase, an enzyme that does have proofreading activity. We did not find significant differences in the fidelity of either enzyme, even when using optimal... Tipo: Info:eu-repo/semantics/other Palavras-chave: Polymerase chain reaction fidelity; Thermus aquaticus (Taq) DNA polymerase; Thermotoga maritima DNA polymerase. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000001 Registros recuperados: 2 Primeira ... 1 ... Última

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