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Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles BABT
Carneiro,Lara Aparecida Buffoni Campos; Costa-Silva,Tales Alexandre; Souza,Cláudia Regina Fernandes; Bachmann,Luciano; Oliveira,Wanderley Pereira; Said,Suraia.
This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE). The immobilized enzyme retained an activity of about 53.5 % after five reuses, using p-NPP as substrate. Physical adsorption of lipase onto highly deacetylated CHM showed 46.2 % of enzymatic activity and 28.6% of IE. This immobilized derivative did not lose activity up to 80 days of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme immobilization; Chitosan; Cercospora kikuchii; Lipase.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400578
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Antimicrobial activity from endophytic fungi Arthrinium state of Apiospora montagnei Sacc. and Papulaspora immersa BABT
Ramos,Henrique Pereira; Braun,Glaucia Hollander; Pupo,Mônica Tallarico; Said,Suraia.
Papulaspora immersa and Arthrinium state of Apiospora montagnei Sacc. were isolated from the roots of Smallanthus sonchifolius (yacón). The crude extracts from their cultures inhibited the growth of Staphylococcus aureus, Kocuria rhizophila, Pseudomonas aeruginosa and Escherichia coli. The more relevant results were observed in the ethyl acetate extract from P immersa against P aeruginosa (90 µg/mL) and ethyl acetate extract from Arthrinium state of A montagnei Sacc. against P aeruginosa (160 µg/mL). The two endophytic fungi isolated from yacón roots as well as their antimicrobial activity detected in the crude extracts cultures were being reported for the first time.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arthrinium state of Apiospora montagnei; Papulaspora immersa; Smallanthus sonchifolius; Yacón.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300017
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Effect of benzene compounds from plants on the growth and hyphal morphology in Neurospora crassa BJM
Neves,Fabrícia Mendonça; Kawano,Cristina Yoshiko; Said,Suraia.
The effects of the benzene compounds from plants, respectively cinnamic acid, coumaric acid, ferulic acid, caffeic acid, and cinnamic aldehyde on growth and hyphal morphology of Neurospora crassa, were investigated. Cinnamic acid, ferulic acid and cinnamic aldehyde inhibited colony growth, but produced no visible alterations on hyphae. Caffeic acid and coumaric acid did not inhibit growth, but changed hyphal morphology. The results suggest that caffeic and coumaric acids probably affect polarity maintenance (the continued deposition of wall material at the extending tip), while cinnamic aldehyde, ferulic and cinnamic acids decrease growth rate, but did not change hyphal polarity. The actin cytoskeleton and the Spitzenkörper appeared diffuse and not clearly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cinnamic acid; Ferulic acid; Cinnamic aldehyde.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200016
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Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans BJM
Barense,Renata I.; Chellegatti,Maria Angélica dos S.C.; Fonseca,Maria José V.; Said,Suraia.
Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exopolygalacturonase; Pectinase; Polygalacturonase; Penicillium frequentans.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400014
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