| The Prasinophyceae Tetraselmis suecica Butcher, was cultivated at a density of 2 to 3 million cells per ml and was concentrated with a decanting centrifugal extractor at 1 m3/hr flow. The samples, obtained in the form of a paste, were diluted to 50 x 10(6) cells per ml and mixed with two cryoprotective agents such as glycerol (10, 20, 30%) and dimethyl sulfoxide (DMSO, 5, 10, 15%), then frozen and stored a t -25 C. After a 1 month period, results by decreasing order of viability areas follows : glycerol, l0%, 20%, DMSO 15%, glycerol 10%, a starting culture of 0.69 x 10(6) cells per ml in 500 ml sea water enriched with Conway stock solution reached 5.49 x 10(6) cells per ml culture a t the end of 4 days. The aim of these experiments was to obtain a good... |
