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Irradiation effect on in vitro organogenesis, callus growth and plantlet development of Gerbera jamesonii Horticultura Brasileira
Hasbullah,Nor A; Taha,Rosna M; Saleh,Azani; Mahmad,Noraini.
The present work was carried out to study the effects of gamma irradiation on in vitro growth of explants, callus and the formation of shoots and plantlets. Irradiation is known to exhibit or inhibit the differentiation of cells and growth of plants in vitro, which helps in producing new plant varieties. Gamma irradiation is one of the physical mutagens that are widely used for mutation breeding. A gradual decline was observed in the number of shoots regenerated from irradiated petiole explants compared to control. Numbers of shoots regenerated from irradiated petiole explant cultured on Murashige & Skoog medium supplemented with 2.0 mg L-1 BAP and 0.5 mg L-1 NAA was reduced to 6.6±0.9 from 7.5±0.4 (control) when explants were exposed to 20 Gray of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gerbera jamesonii; Ornamental; Gamma irradiation; Explant; Callus regeneration; Plant growth regulator; MS medium.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000200012
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Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation BABT
Hasbullah,Nor Azlina; Taha,Rosna Mat; Saleh,Azani; Mohamed,Normadiha.
In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gerbera jamesonii; Gamma irradiation; Callus; In vitro; Chlorophyll; Soluble protein.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300012
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