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| Salvarani,F.M.; Lobato,Z.I.P.; Assis,R.A.; Lima,C.G.R.D.; Silva,R.O.S.; Pires,P.S.; Lobato,F.C.F.. |
| Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seroneutralization; Mouse; Guinea pig; Cell culture; Vaccine. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002 |
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| Assis,R.A.; Lobato,F.C.F.; Lobato,Z.I.P.; Camargos,M.F.; Nascimento,R.A.P.; Vargas,A.P.C.; Salvarani,F.M.; Uzal,F.A.. |
| Padronizou-se uma técnica de reação em cadeia da polimerase múltipla (PCR multiplex) para detecção de Clostridium chauvoei e Clostridium septicum em culturas puras. Foram utilizados pares de iniciadores para segmentos específicos dos genes que codificam a flagelina de C. chauvoei e a toxina alfa de C. septicum. Para avaliaçã o da PCR multiplex, foram testados 16 isolados clínicos de C. chauvoei e 15 isolados de C. septicum provenientes de ruminantes, quatro sementes vacinais de cada um desses agentes. Amostras de referência de ambos os microrganismos foram usadas como controle. Para avaliar a especificidade, DNAs genômicos dos seguintes microrganismos foram usados: C. sordellii, C. novyi tipo A, C. novyi tipo B, C. perfringens tipo A, C. haemolyticum, C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PCR multiplex; Identificação; Clostridium chauvoei; Clostridium septicum. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200003 |
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| Silva,R. O. S.; Salvarani,F.M.; Assis,R.A.; Martins,N.R.S.; Pires,P.S.; Lobato,F.C.F.. |
| Clostridium perfringens is a normal inhabitant of the intestinal tract of chickens as well as a potential pathogen that causes necrotic enteritis and colangio hepatitis. The minimum inhibitory concentration (MIC) of seven different compounds used for therapy, growth promotion or prevention of coccidiosis was determined by agar dilution method for 55 C. perfringens strains isolated from the intestines of broiler chickens. All strains showed high susceptibility to penicillin, avilamycin, monensin and narasin. Only 7.3% of the strains showed an intermediated sensitivity to lincomycin, and 49 (89.1%) were considered susceptible. For tetracycline and bacitracin, 41.8% and 47.3% of strains, respectively, were considered resistant. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Necrotic enteritis; Growth promoters; Anticoccidials; Antibiotics. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200010 |
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