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	Provedor de dados BABT (2) Autor Assis,Helena Cristina da Silva de (2) Klemz,Claudio (2) Salvo,Ligia Maria (2) Bainy,Afonso Celso Dias (1) Bastos Neto,Jayme da Cunha (1) Calegari,Rossana (1) Nicareta,Lilian (1) Truppel,Jessé Henrique (1) Mais... Palavra-chave Ancistrus multispinis (2) Biomarker (2) Cytochrome P450 (2) Fish (2) Deltamethrin (1) Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2009 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) BABT Klemz,Claudio; Salvo,Ligia Maria; Bastos Neto,Jayme da Cunha; Bainy,Afonso Celso Dias; Assis,Helena Cristina da Silva de. Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochrome P450; Fish; Ancistrus multispinis; Biomarker. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015 Biochemical biomarkers of exposure to deltamethrin in freshwater fish, Ancistrus multispinis BABT Assis,Helena Cristina da Silva de; Nicareta,Lilian; Salvo,Ligia Maria; Klemz,Claudio; Truppel,Jessé Henrique; Calegari,Rossana. This study aimed to determine the effect of sublethal doses of deltamethrin, using biochemical biomarkers as activities of cholinesterase (ChE), ethoxyresorufin-O-deethylase (EROD) and the Na+K+- ATPase and levels of total cytochrome P450 (CYP450). Fishes received sublethal doses of deltamethrin and were sacrificed after 96 h of exposure. Samples of gills, heart, brain, liver and muscle were collected for enzymatic analyses. Deltamethrin inhibited the activity of the gills and heart Na+ K+-ATPase, induced the liver total CYP450, as well as the liver EROD activity. The activity of the ChE was not inhibited by deltamethrin. Deltamethrin altered the hepatic metabolism and the normal ionic flux in Ancistrus multispinis. Tipo: Info:eu-repo/semantics/article Palavras-chave: Deltamethrin; Cytochrome P450; Fish; Ancistrus multispinis; Biomarker. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600012 Registros recuperados: 2 Primeira ... 1 ... Última

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