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BOX-PCR-based identification of bacterial species belonging to Pseudomonas syringae: P. viridiflava group 74
Marques,Abi S.A.; Marchaison,Anne; Gardan,Louis; Samson,Régine.
The phenotypic characteristics and genetic fingerprints of a collection of 120 bacterial strains, belonging to Pseudomonas syringae sensu lato group, P. viridiflava and reference bacteria were evaluated, with the aim of species identification. The numerical analysis of 119 nutritional characteristics did not show patterns that would help with identification. Regarding the genetic fingerprinting, the results of the present study supported the observation that BOX-PCR seems to be able to identify bacterial strains at species level. After numerical analyses of the bar-codes, all pathovars belonging to each one of the nine described genomospecies were clustered together at a distance of 0.72, and could be separated at genomic species level. Two P. syringae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas syringae; Phenotypic characters; Genomospecies; BOX-PCR; Bacterial identification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100019
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Population dynamics of Pseudomonas savastanoi pv. phaseolicola in bean, throughout the epiphytic and pathogenic phases 86
Marques,Abi Soares dos Anjos; Samson,Régine.
Abstract: The objective of this work was to monitor traits of the life cycle of Pseudomonas savastanoi pv. phaseolicola, in order to better understand the outbreak of bean halo blight, originating from a bacterial population in asymptomatic plants. Five experiments were conducted in the field, in greenhouses, and in humidity chambers. Changes in population size were evaluated in three field plantings, by introducing the bacteria in contamination focal points and observing the weather conditions favoring an outbreak. The dispersion of the bacteria in the field was followed by isolation and Bio-PCR analysis. Two assays were conducted in greenhouses and humidity chambers to evaluate the effect of leaf age on disease expression and the relationship between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Bio-PCR; Epidemiology; Halo blight; Plant quarantine; Weather conditions..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500623
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