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Papazian-Cabanas,Rodrigo M.; Araújo,Eduardo J.A.; Silva,Aristeu V. da; Sant'Ana,Débora M.G.. |
The effects of acute and chronic infection caused by Toxoplasma gondii on duodenal myenteric neurons were analyzed. Eighteen rats were assigned into four groups: Acute Control Group (ACG, n=4); Acute Experimental Group (AEG, n=4); Chronic Control Group (CCG, n=5); and Chronic Experimental Group (CEG, n=5). Rats from the AEG and CEG were inoculated orally with 105 genotype III (BTU-II strain) tachyzoites of T. gondii isolated from a dog with neurological signs. Acute groups were killed after 24 hours after the inoculation and the chronic groups after 30 days. Whole-mount from the duodenum were stained with Giemsa. The population density of myenteric neurons, as well the body cell, nuclear and cytoplasmic area were analyzed. Both acute and chronic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric nervous system; Infection; Parasite; Toxoplasmosis. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300016 |
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Sant'Ana,Débora M.G.; Araújo,Eduardo J.A.; Ramos,Diego H.; Hermes-Uliana,Catchia; Natali,Maria Raquel M.. |
The effects of severe protein malnutrition (4%) on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts - 5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus®software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric nervous system; Malnutrition; Myenteric plexus; Nitrergic neurons. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300022 |
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