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| Nascimento,Demétrius Fernandes do; Moraes,Manoel Odorico de; Bezerra,Fernando Antônio Frota; Pontes,Andréa Vieira; Uchoa,Célia Regina Amaral; Moraes,Renata Amaral de; Leite,Ismenia Osório; Santana,Gilmara Silva de Melo; Santana,Ana Paula Macedo; Leite,Ana Lourdes Almeida e Silva; Pedrazzoli Júnior,José; Moraes,Maria Elisabete Amaral de. |
| To develop and validate a rapid, specific and highly sensitive method to quantify nimodipine in human plasma using dibucaine as the internal standard (IS). The analyte and IS were extracted from plasma samples by liquid-liquid extraction using hexane-ethyl acetate (1:1 v/v). The chromatographic separation was performed on a Varian® Polaris C18 analytical column (3 μm, 50 x 2.0 mm) and pre-column SecurityguardTM C18 (4.0 x 3.0 mm) with a mobile phase of Acetonitrile-Ammonium acetate 0.02 ml/L (80:20v/v). The method had a chromatographic run time of 4.5 min and linear calibration curve over the range of 0.1- 40 ng/mL (r > 0.9938). The limit of quantification was 100 pg/mL. Acceptable precision and accuracy were obtained for concentrations over the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nimodipine/determination in plasma; Nimodipine/pharmacokinetic profile; Nimodipine/quantitative analysis; Pharmacokinetics/method validation. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400008 |
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