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| Silva,Paulo da; Santos,Adolfo Carlos Barreto; Sato,Daisy Nakamura; Silva,Jaqueline Otero; Medeiros,Marta Inês Cazentini; Carneiro,Ana Maria Machado; Leite,Sergio Roberto de Andrade; Leite,Clarice Queico Fujimura. |
| INTRODUCTION: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics. OBJECTIVE: This study aimed to characterize bacterial isolates from sputum samples suggestive of R. equi. METHODS: The phenotypic identification included biochemical assays; thin-layer chromatography (TLC) and polymerase chain reaction (PCR) were used for genotypic identification. RESULTS: Among 78 Gram-positive and partially acid-fast bacilli... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymerase chain reaction; Rhodococcus equi; Mycolic acids. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500001 |
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| Prince,Karina Andrade de; Sordi,Renata; Pavan,Fernando Rogério; Santos,Adolfo Carlos Barreto; Araujo,Angela R.; Leite,Sergio R.A.; Leite,Clarice Q. F.. |
| Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phomopsis stipata; Antimycobacterial activity; Mycobacterium tuberculosis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100024 |
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| Siqueira,Vera Lúcia Dias; Cardoso,Rosilene Fressatti; Pádua,Rubia Andreia Falleiros de; Caleffi-Ferracioli,Katiany Rizzieri; Helbel,Cesar; Santos,Adolfo Carlos Barreto; Aoki,Elisabeth Eyko; Nakamura,Celso Vataru. |
| In Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Paraná, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-β-lactamase (MBL) was detected using a disk approximation test. Genotyping was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas aeruginosa/antimicrobial resistance profile; Pseudomonas aeruginosa/genetic study; Acinetobacter spp./antimicrobial resistance profile; Acinetobacter spp./genetic study; Antimicrobial resistance; Bacterial typing. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100006 |
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