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Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine) Genet. Mol. Biol.
Schlögl,Paulo Sérgio; Santos,André Luis Wendt dos; Vieira,Leila do Nascimento; Floh,Eny Iochevet Segal; Guerra,Miguel Pedro.
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz.,ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semiquantitative RT-PCR during embryo development...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed development; Brazilian Pine; Embryogenesis-regulating genes; Zygotic embryogenesis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100025
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Biochemical and morphological changes during the growth kinetics of Araucaria angustifolia suspension cultures BABT
Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Maraschin,Marcelo; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 µM 2,4-dichlorophenoxyacetic acid, 1 µM 6-benzylaminopurine and 1 µM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive reduction during culture time. For both the embryogenic cultures a preferential uptake of glucose in the late stages of cell growth kinetics was observed. The extracellular protein content was similar for both the embryogenic cultures. Acetocarmine and Evan's blue double stain showed major differences for early somatic embryo organisation, in which only the embryogenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucariaceae; Glucose; PH changes; Protein; Somatic embryogenesis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300001
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Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) BABT
Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Vidor,Mário; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucaria angustifolia; Conifer; Somatic embryogenesis; Picea abies; Embryo maturation; Pinus sp..
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015
Registros recuperados: 3
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