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| Nascimento,D.V.; Lemes,E.M.B.; Queiroz,J.L.S.; Silva Jr.,J.G.; Nascimento,H.J.; Silva,E.D.; Hirata Jr.,R.; Dias,A.A.S.O.; Santos,C.S.; Pereira,G.M.B.; Mattos-Guaraldi,A.L.; Armoa,G.R.G.. |
| The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently available. In the present study, the genomic DNA of the Corynebacterium diphtheriae Park Williams 8 (PW8) vaccine strain was used as a template for PCR amplification of the dtb gene. After amplification, the dtb gene was cloned and expressed in competent Escherichia coli M15™ cells using the expression vector pQE-30™. The lysate obtained from transformed E. coli cells containing the rDTB PW8 was clarified by centrifugation and purified by affinity chromatography. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragment B; Diphtheria toxin; Diphtheria; Dtb gene; E. coli gene expression; Immobilized metal affinity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500007 |
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