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Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection BJM
Lima-Neto,Reginaldo; Santos,Cledir; Lima,Nelson; Sampaio,Paula; Pais,Célia; Neves,Rejane P..
Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BRCs; Candida; Clinical yeasts; Culture collections; MALDI-TOF MS.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200020
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