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| Sobrinho,E.M.; Almeida,A.C.; Brandi,I.V.; Colen,F.; Lobato,F.C.F.; Cangussu,A.S.R.; Santos,H.O.; Quintilio,W.; Sari,R.S.. |
| This study aimed to assess and standardize the ELISA and modified ToBI test in vitro methods in order to verify the potency of epsilon toxicoid in comparison with the in vivo TCP method. The following epsilon toxoids were used: NIBSC standard from batches 375/07, 532/08, 551/08, 373/07 and 378/07. These were evaluated using a TCP test, ELISA and ToBI tests. The results indicate that the correlation ratio between the dilutions of standard NIBSC toxicoid and absorbance values of 89.44% obtained with the ELISA method support the use of the curve to evaluate epsilon toxoids. However, it was observed that the absorbance values were similar for all toxoids, thus presenting no significant difference between higher and lower concentration toxoids. For the ToBI... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epsilon toxicoid; Clostridiosis; In vitro alternative method. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300713 |
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