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| Silva,Rodrigo Otávio Silveira; Santos,Renata Lara Resende; Pires,Prhiscylla Sadanã; Pereira,Luiz Carlos; Pereira,Silvia Trindade; Duarte,Marina Carvalho; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria. |
| The objective of this study was to detect C. difficileA/B toxins and to isolate strains of C. perfringensand C. difficile from diarrheic and non-diarrheic dogs in Brazil. Stool samples were collected from 57 dogs, 35 of which were apparently healthy, and 22 of which were diarrheic. C. difficileA/B toxins were detected by ELISA, and C. perfringensand C. difficilewere identified by multiplex PCR. C. difficileA/B toxins were detected in 21 samples (36.8%). Of these, 16 (76.2%) were from diarrheic dogs, and five (23.8%) were from non-diarrheic dogs. Twelve C. difficile strains (21.1%) were isolated, of which ten were A+B+and two were A-B-. All non-toxigenic strains were isolated from non-diarrheic animals. The binary toxin gene cdtBwas found in one strain,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial diarrhea; Small animals; Colitis; Enteritis; Canine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100019 |
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| Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Cruz Júnior,Eduardo Coulaud da Costa; Pires,Prhiscylla Sadanã; Santos,Renata Lara Resende; Assis,Ronnie Antunes de; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria. |
| Clostridium difficile has emerged as a major cause of neonatal colitis in piglets, displacing classic bacterial pathogens. However, there is no information regarding the distribution of this microorganism in pig farms in Brazil. In the present study, the presence of toxins A/B and of C. difficile strains in stool samples from 60 diarrheic or non-diarrheic newborn piglets (one to seven days old), from 15 different farms, was studied. The presence of toxins A/B was detected by ELISA and PCR was used to identify toxin A, toxin B and binary toxin gene in each isolated strain. C. difficile A/B toxins were detected in ten samples (16.7%). Of these, seven were from diarrheic and three were from non-diarrheic piglets. C. difficile was recovered from 12 out of 60... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neonatal diarrhea; Clostridium difficile; Enteritis; Colitis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800021 |
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