|
|
|
|
|
| Lima,C.C.V.; Ayres,M.C.C.; Pinheiro,R.R.; Costa,J.N.; Andrioli,A.; Souza,T.S.; Azevedo,D.A.A.; Santos,V.W.S.; Araújo,J.F.; Sousa,A.L.M.; Peixoto,R.M.; Damasceno,E.M.; Costa Neto,A.O.. |
| ABSTRACT With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Regarding the rams, eight semen collections were made per animal, during one year of experimentation, totaling 32 samples. The milk and semen samples were submitted to DNA extraction and the nested polymerase chain reaction test (nPCR) to detect CLV proviral DNA. Eight (16%) of the milk samples were positive in nPCR originating from two ewes. Only one (3.12%) semen sample was positive. The amplification products were sequenced, and were confirmed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lentiviruses; Transmission; Cross infection. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200391 |
| |
|
|
| Sousa,A.L.M.; Pinheiro,R.R.; Araújo,J.F.; Santos,V.W.S.; Azevedo,D.A.A.; Peixoto,R.M.; Souza,V.; Andrioli,A.; Damasceno,E.M.; Dantas,T.V.M.; Teixeira,M.F.S.. |
| ABSTRACT The aim of this study was to evaluate in vitro and in vivo the effect of sodium dodecyl sulfate (SDS) on the caprine lentivirus (CLV) in colostrum and milk. This was performed to develop a practical and efficient method of blocking the lactogenic transmission of the virus. In the in vitro experiment, colostrum and milk were treated with 0.25%; 0.50% and 1% SDS. Then, somatic cells of colostrum and milk were submitted to co-culture with caprine synovial membrane cells (CSM). In the in vivo test, goats were fed with colostrum and milk provided from CLV-positive goats treated with SDS in the same concentrations used in the in vitro experiment. Animals were tested by nested polymerase chain reaction (nPCR) and Western blot (WB) assays. In the in vitro... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monocyte-phagocytic system; Viral inactivation; Surfactant; Small ruminant lentiviruses. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501459 |
| |
|
|
|
