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| Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Santos,Vanderley Borges dos; Souza Sobrinho,Fausto; Davide,Lisete Chamma. |
| Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Colchicine; Nuclear DNA content. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000600001 |
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| Mendonça,Márcia Silva de; Beber,Paulo Márcio; Nascimento,Francisca Silvana Silva do; Santos,Vanderley Borges dos; Marinho,José Tadeu. |
| ABSTRACT Cowpea is a legume with ample plasticity, versatility and nutritional potential. It is a species widely used as a source of income and subsistence for small farmers in several Brazilian states, among them Acre. Due to the different varieties found in the State, it is the target of studies aiming at its genetic improvement. Thus, as one of the first stages of its improvement, it was aimed to determine the importance and correlations of characters for diversity and selection in traditional varieties of cowpea. The experiment was carried in completely randomized design, with plots consisting of four vase with capacity to 15.7 L, with one plant each and two replicates. The characteristics evaluated were: days for emergence, flowering (days), plant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vigna unguiculata. Phenotypic; Genotypic and environmental correlations. Principal components. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000200267 |
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