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	Provedor de dados BJM (3) Autor Saridakis,Halha Ostrensky (3) Arantes,Olívia Márcia Nagy (1) Barouni,Abdulmonam Saied (1) Dias,Angela M.G. (1) Furlaneto,Luciana (1) Kobayashi,Renata K. T. (1) Vidotto,Marilda C. (1) Vidotto,Marilda Carlos (1) Mais... Palavra-chave Bacillus thuringiensis (1) Conjugation (1) Diarrhoea (1) Digoxigenin-labelled DNA probes (1) EPEC (1) Enteropathogenic Escherichia coli (1) Microbial ecology (1) Molecular diagnosis (1) Multiprimer-PCR (1) Mycobacterium tuberculosis (1) Serotypes (1) Virulence factors (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2004 (1) 2000 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Molecular identification of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea in Londrina, Parana, Brazil BJM Kobayashi,Renata K. T.; Saridakis,Halha Ostrensky; Dias,Angela M.G.; Vidotto,Marilda C.. In this work, the prevalence of enteropathogenic Escherichia coli (EPEC) in children in Londrina-PR, Brazil, was evaluated by means of digoxigenin-labelled DNA probes which identify the plasmid responsible for EPEC adherence factor (EAF), and virulence genes for EPEC as bundle-forming pilus (bfp) and E. coli attaching-effacing factor (eae). In addition, the isolated strains were serotyped and tested for adherence to HEp-2 cells. From 102 children with diarrhoea, 19 strains hybridized with at least one probe, and eleven of them were identified as typical EPEC because they hybridized with the three probes used, showed a localized adherence (LA) pattern, and presented no genes for enterotoxins (ST and LT) or invasion as detected by PCR. Six of the typical... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Digoxigenin-labelled DNA probes; Virulence factors; Serotypes; Diarrhoea. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400007 Survival and conjugal transfer between Bacillus thuringiensis strains in aquatic environment BJM Furlaneto,Luciana; Saridakis,Halha Ostrensky; Arantes,Olívia Márcia Nagy. Field and laboratory studies were conducted to assess the survival of cells and spores and plasmid transfer between Bacillus thuringienis strains in aquatic environment. Results indicated that cells and spores of B. thuringiensis can survive for 10 days in water, without altering their number. The sporulation process began after 12-15 hours of inoculation of water. B. thuringiensis was able to transfer conjugative plasmids in the aquatic environment. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; Conjugation; Microbial ecology. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400001 Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction BJM Barouni,Abdulmonam Saied; Saridakis,Halha Ostrensky; Vidotto,Marilda Carlos. We investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Multiprimer-PCR; Molecular diagnosis. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004 Registros recuperados: 3 Primeira ... 1 ... Última

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