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	Provedor de dados BABT (2) Electron. J. Biotechnol. (2) BJM (1) Ciência Rural (1) Autor Sarubbo,Leonie Asfora (6) Campos-Takaki,Galba Maria de (2) Porto,Ana Lúcia Figueiredo (2) Tambourgi,Elias Basile (2) Andrade,Samara Alvachian Cardoso (1) Andrade,Vânia Sousa (1) Bezerra de Souza Sobrinho,Humberto (1) Campos-Takaki,Galba Maria (1) Cortez,Neila Mello Santos (1) Diniz Rufino,Raquel (1) Farias,Charles B.B. (1) Ferreira Silva,Aline (1) Figueiredo Porto,Ana Lúcia (1) Fukushima,Kasutaka (1) Gomes Souza,José Edson (1) Lima-Filho,José Luís (1) Melo,Homero José Farias de (1) Miyaji,Makoto (1) Moreira,Keila Aparecida (1) Moura Luna,Juliana (1) Mais... Palavra-chave Aqueous two-phase system (1) Aqueous two-phase systems (1) Ascorbic oxidoreductase (1) Bioreactor (1) Biosurfactant (1) Candida sphaerica (1) Cashew-nut tree gum (1) D-glucose (1) Efficiency (1) Extracellular proteases (1) Industrial residues (1) Ine serum albumin (1) Mass transfer coefficient (1) Microbial surfactant (1) Microbiological analysis (1) Mucor circinelloides (1) Nanoscale materials (1) Perforated Rotating Disc contactor (1) Protein partition (1) Pseudomonas aeruginosa (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Journal article (2) Ano 2019 (1) 2014 (1) 2013 (1) 2004 (2) 2002 (1) País Brazil (4) Chile (2) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor BABT Porto,Ana Lúcia Figueiredo; Sarubbo,Leonie Asfora; Moreira,Keila Aparecida; Melo,Homero José Farias de; Lima-Filho,José Luís; Campos-Takaki,Galba Maria; Tambourgi,Elias Basile. A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Efficiency; Mass transfer coefficient; Perforated Rotating Disc contactor; Aqueous two-phase system; Ascorbic oxidoreductase. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019 Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) BABT Sarubbo,Leonie Asfora; Oliveira,Luciana Alves de; Porto,Ana Lúcia Figueiredo; Campos-Takaki,Galba Maria de; Tambourgi,Elias Basile. The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aqueous two-phase systems; Ine serum albumin; Cashew-nut tree gum; Protein partition. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003 Production of extracellular proteases by Mucor circinelloides using D-glucose as carbon source / substrate BJM Andrade,Vânia Sousa; Sarubbo,Leonie Asfora; Fukushima,Kasutaka; Miyaji,Makoto; Nishimura,Kazuko; Campos-Takaki,Galba Maria de. Recently, some Mucorales species have been reported as protease producers. The production of extracellular proteases by Mucor circinelloides using glucose as substrate was studied. Experiments were carried out with different D-glucose concentrations (40, 60 and 80 g/L). Biomass, pH and protease activity were determined. Although biomass production had reached best yields for the medium containing D-glucose in a concentration of 80 g/L, the enzymatic production was higher when the substrate concentration was reduced to 40 g/L. The yield factor for product on cell growth and the yield factor for product on carbon substrate were higher when the microorganism grew in medium containing 40 g/L glucose. The kinetics parameters suggest that this strain seems to be... Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular proteases; D-glucose; Substrate; Mucor circinelloides. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200002 Optimization of the osmotic dehydration of ginger Ciência Rural Siqueira,Leonardo Pereira de; Sarubbo,Leonie Asfora; Shinohara,Neide Kazue Sakugawa; Valencia,Marcela Sarmento; Cortez,Neila Mello Santos; Andrade,Samara Alvachian Cardoso. ABSTRACT: The present research aimed to optimize the process of osmotic dehydration (OD) of ginger with hypertonic sucrose solution employing response surface methodology. A 23 experimental planning was carried out and 17 experimental assays were performed based on three independent variables (temperature, concentration of the osmotic solution and immersion time) and three dependent variables (moisture loss (ML), solids gain (SG) and dehydration efficiency index (DEI)). The selected assay conditions exhibited the preferred DEI value (the highest ML and lower SG), which were considered in the optimization. Assay 16 demonstrated to be the most favorable conditions for the osmotic dehydration of ginger (DEI =1.61) at 40 40 °C, 50 °Brix and 90 minutes of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Zingiber officinale Roscoe; Water loss; Microbiological analysis. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001000754 Assessment of toxicity of a biosurfactant from Candida sphaerica UCP 0995 cultivated with industrial residues in a bioreactor Electron. J. Biotechnol. Bezerra de Souza Sobrinho,Humberto; de Luna,Juliana Moura; Rufino,Raquel Diniz; Figueiredo Porto,Ana Lúcia; Sarubbo,Leonie Asfora. Background: The aim of the present study was to propose a low-cost method for the production of a biosurfactant by the yeast Candida sphaerica and assess its toxicity and phytotoxicity. The medium was formulated with distilled water supplemented with residue from a soy oil refinery (5%) and corn steep liquor (2.5%) as substrates. These two products were the sources of carbon and nitrogen as well as mineral elements to encourage the growth of the microorganism and production of a biosurfactant. Results: The isolated biosurfactant yield was 6.364 g/l. The biosurfactant exhibited an excellent ability to reduce surface tension (26 mN/m) and demonstrated no toxicity against seeds of Brassica oleracea, Chicoria intybus and Solanum gilo or the micro crustacean... Tipo: Journal article Palavras-chave: Biosurfactant; Bioreactor; Candida sphaerica; Industrial residues; Toxicity. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400004 Synthesis of silver nanoparticles using a biosurfactant produced in low-cost medium as stabilizing agent Electron. J. Biotechnol. Farias,Charles B.B.; Ferreira Silva,Aline; Diniz Rufino,Raquel; Moura Luna,Juliana; Gomes Souza,José Edson; Sarubbo,Leonie Asfora. Background A biosurfactant produced by Pseudomonas aeruginosa cultivated in a low-cost medium formulated with 2.5% vegetable oil refinery residue and 2.5% corn steep liquor and distilled water was employed to stabilize silver nanoparticles in the liquid phase. The particles were initially synthesized using NaBH4 as reducing agent in biosurfactant reverse micelles and were extracted from the micellar solution to disperse in heptane. Results A silver particle size in the range of 1.13 nm was observed. The UV-vis absorption spectra proposed that silver nanoparticles could be formed in the reverse micelles and relatively stabilized for at least 3 months without passivator addition. The Transmission Electron Microscope (TEM) shows that the silver nanoparticles... Tipo: Journal article Palavras-chave: Microbial surfactant; Nanoscale materials; Pseudomonas aeruginosa. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300003 Registros recuperados: 6 Primeira ... 1 ... Última

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