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The post-transcriptional gene silencing pathway in Eucalyptus Genet. Mol. Biol.
Sassaki,Flávio Tetsuo; Campos-Pereira,Tiago; Maia,Ivan de Godoy.
Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene silencing; PTGS; RNA silencing; EST; Eucalyptus.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400003
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Putative metabolic pathway of mannitol and sorbitol and in sugarcane Scientia Agricola
Marino,Celso Luís; Leite,Susi Meire Maximino; Farro,Ana Paula Cazerta; Sassaki,Flávio Tetsuo; Campos,Henrique Luis Veronezi de; Coscrato,Virgínia Elias.
Until the mid 1950s, boron was believed to play an important role in the transport of sugars in plants. However, boron actually depends on sugar alcohols to be taken up by the plant. In some cases, the main sugars involved in this process are sorbitol and mannitol which form stable complexes with boron. In this study, the sequences of the SugarCane EST Genome Project (SUCEST) database were searched for enzymes involved in the metabolism of these sugars by comparing them with enzymes from other organisms. Eighteen contigs from sugarcane (Saccharum sp.) presented high similarity with 11 enzymes involved in the putative biosynthetic pathway of sorbitol and mannitol from fructose in sugarcane. Seven of these contigs had high homology with sequences deposited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Boron; Expressed sequence tags (ESTs); Polyols; Sugar alcohol.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400017
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