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Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis Rev. Ciênc. Agron.
Victório,Cristiane Pimentel; Lage,Celso Luiz Salgueiro; Sato,Alice.
The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical), a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Shoots (Plants); Plant-tissue culture; Plant-growth regulators.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000300017
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Developmental effects of additional ultraviolet a radiation, growth regulators and tyrosine in Alternanthera brasiliana (L.) Kuntze cultured in vitro BABT
Silva,Nina Cláudia Barboza; Macedo,Andrea Furtado; Lage,Celso Luís Salgueiro; Esquibel,Maria Apparecida; Sato,Alice.
Tissue cultures of Alternanthera brasiliana (L.) Kuntze were treated with different growth regulators (Kinetin and 2,4-D), tyrosine and Ultraviolet A radiation (UV-A; 320 - 400 nm) to investigate their effects on development and pigment production. Nodal segments of plantlets grown from seeds were inoculated in all tested media and different light conditions. After eight weeks, this material was used to evaluate biomass, chlorophyll and betacyanin production. The Murashige and Skoog (MS) + kinetin medium resulted in development of approximately four shoots/explant. This medium plus white light was the best combination for micropropagation with the highest rooting percentage and betacyanin production. Plantlets grown under UV-A illumination reduced biomass...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Betacyanin; Chlorophyll; Morphogenesis; Tissue Culture.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600014
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