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	Provedor de dados BJM (4) Rev. Microbiol. (1) Autor Sato,Hélia H. (5) Kawaguti,Haroldo Y. (2) Ferracini-Santos,Luciana (1) Fleuri,Luciana F. (1) Orsi,Daniela C. (1) Soares,Giselle A. M. (1) Soares,Giselle A.M. (1) Mais... Palavra-chave Cellulosimicrobium cellulans (2) Killer toxin (2) Killer yeast (2) Saccharomyces cerevisiae (2) Actinomycete (1) Alkaline protease (1) Cell immobilization (1) Chitinase (1) Fungal lysis (1) Glucosyltransferase (1) Klebsiella sp (1) Medium optimization (1) Palatinose (1) Protoplasts (1) Response surface (1) Response surface methodology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Ano 2009 (3) 2000 (1) 1999 (1) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Killer toxin of Saccharomyces cerevisiae Y500-4L active against Fleischmann and Itaiquara commercial brands of yeast Rev. Microbiol. Soares,Giselle A.M.; Sato,Hélia H.. The strain Saccharomyces cerevisiae Y500-4L, previously selected from the must of alcohol producing plants and showing high fermentative and killer capacities, was characterized according to the interactions between the yeasts and examined for curing and detection of dsRNA plasmids, which code for the killer character. The killer yeast S. cerevisiae Y500-4L showed considerable killer activity against the Fleischmann and Itaiquara commercial brands of yeast and also against the standard killer yeasts K2 (S. diastaticus NCYC 713), K4 (Candida glabrata NCYC 388) and K11 (Torulopsis glabrata ATCC 15126). However S. cerevisiae Y500-4L showed sensitivity to the killer toxin produced by the standard killer yeasts K8 (Hansenula anomala NCYC 435), K9 (Hansenula... Tipo: Info:eu-repo/semantics/article Palavras-chave: Killer yeast; Saccharomyces cerevisiae; Killer toxin. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300012 Production of alkaline protease from Cellulosimicrobium cellulans BJM Ferracini-Santos,Luciana; Sato,Hélia H.. Cellulosimicrobium cellulans is one of the microorganisms that produces a wide variety of yeast cell wall-degrading enzymes, β-1,3-glucanase, protease and chitinase. Dried cells of Saccharomyces cerevisiae were used as carbon and nitrogen source for cell growth and protease production. The medium components KH2PO4, KOH and dried yeast cells showed a significant effect (p<0.05) on the factorial fractional design. A second design was prepared using two factors: pH and percentage of dried yeast cells. The results showed that the culture medium for the maximum production of protease was 0.2 g/l of MgSO4.7H2O, 2.0 g/l of (NH4)2SO4 and 8% of dried yeast cells in 0.15M phosphate buffer at pH 8.0. The maximum alkaline protease production was 7.0 ± 0.27 U/ml... Tipo: Info:eu-repo/semantics/article Palavras-chave: Response surface; Medium optimization; Alkaline protease; Cellulosimicrobium cellulans; Actinomycete. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100008 Glucosyltransferase production by Klebsiella sp. K18 and conversion of sucrose to palatinose using immobilized cells BJM Orsi,Daniela C.; Kawaguti,Haroldo Y.; Sato,Hélia H.. The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL-1) was achieved using the optimized medium composed by sugar cane molasses (80 g L-1), bacteriological peptone (7 g L-1) and yeast extract (20 g L-1), after 8 hours of fermentation at 28°C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration (25-45%)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell immobilization; Glucosyltransferase; Klebsiella sp; Palatinose; Response surface methodology. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100010 Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 BJM Fleuri,Luciana F.; Kawaguti,Haroldo Y.; Sato,Hélia H.. This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts. Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Cellulosimicrobium cellulans; Fungal lysis; Protoplasts. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026 Characterization of the Saccharomyces cerevisae Y500-4L killer toxin BJM Soares,Giselle A. M.; Sato,Hélia H.. The strain Saccharomyces cerevisiae Y500-4L, selected from the must of alcohol producing plants, liberates a toxin which is lethal to the commercial yeast produced by Fleischmann Royal Nabisco and other strains of yeast. This toxin was characterized, and the maximum production was obtained after 24 hours of incubation at 25ºC in YEPD medium. The maximum activity was achieved between pH 4.1 and 4.5 and between 22 and 25ºC and maximum stability in the pH range 3.8 to 4.5 at -10ºC. The killer toxin was inactivated by heating at 40ºC for 1 hour at pH 4.1. After concentration by ultrafiltration of culture supernatants and purification by gel filtration chromatography, the molecular weight of the purified toxin was estimated by SDS-PAGE to be about 18-20 kDa. Tipo: Info:eu-repo/semantics/article Palavras-chave: Killer toxin; Saccharomyces cerevisiae; Killer yeast. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400010 Registros recuperados: 5 Primeira ... 1 ... Última

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