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The role of atomic spectrometry in plant science Organic Eprints
Husted, Søren; Persson, D.P.; Laursen, K.H.; Hansen, T.H.; Pedas, P.; Schiller, M.; Hegelund, J.N.; Schjørring, J.K..
Inductively coupled plasma-mass spectrometry (ICP-MS) is the state-of-the-art technique for multi-elemental analysis of plant tissue. It provides a powerful tool in functional genomics, linking altered elemental profiles of mutants with gene expression and function. In addition, with its unmatched sensitivity, ICP-MS enables characterization of the substrate specificity and regulation of membrane transport proteins. Digestion of plant tissue has traditionally represented a bottleneck due to the low capacity of commercially available equipment. However, recent developments in micro-scaled digestion, combined with semi-quantitative analysis and chemometrics, have enabled high-throughput multi-elemental profiling and multivariate classification of large...
Tipo: Journal paper Palavras-chave: Food systems; Knowledge management.
Ano: 2011 URL: http://orgprints.org/19089/1/Review.pdf
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Simultaneous iron, zinc, sulphur and phosphorus speciation analysis of barley grain tissues using SEC-ICP-MS and IP-ICP-MS Organic Eprints
Persson, Daniel Pergament; Hansen, Thomas Hesselhøj; Laursen, Kristian Holst; Schjørring, J.K.; Husted, Søren.
The increasing prevalence of iron (Fe) and zinc (Zn) deficiencies in human populations worldwide has stressed the need for more information about the distribution and chemical speciation of these elements in cereal products. In order to investigate these aspects, barley grains were fractionated into awns, embryo, bran and endosperm and analysed for Fe and Zn. Simultaneously, phosphorus (P) and sulfur (S) were determined since these elements are major constituents of phytic acid and proteins, respectively, compounds which are potentially involved in Fe and Zn binding. A novel analytical method was developed in which oxygen was added to the octopole reaction cell of the ICP-MS. This approach greatly improved the sensitivity of sulfur, measured as 48SO+....
Tipo: Journal paper Palavras-chave: Food systems.
Ano: 2009 URL: http://orgprints.org/19157/7/19157.pdf
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