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Phytoplankton distribution from Western to Central English Channel, revealed by automated flow cytometry during the summer-fall transition ArchiMer
Louchart, Arnaud; Lizon, Fabrice; Lefebvre, Alain; Didry, Morgane; Schmitt, François G.; Artigas, Luis Felipe.
Automated pulse shape-recording flow cytometry was applied to address phytoplankton spatial distribution, at high frequency, in stratified and well mixed water masses in the Western and Central English Channel during the summer-fall transition. Cytometric pulse shapes derived from optical features of single cells allowed the characterization of eight phytoplankton groups. Abundance and total red fluorescence (chlorophyll a autofluorescence) per group were used to define six phytoplankton communities. Their distribution revealed high spatial heterogeneity. Abundance presented a longitudinal gradient for six over the eight groups and succession of brutal shifts along the cruise. Maximum values were often located near the Ushant front in the Western English...
Tipo: Text Palavras-chave: English Channel; Phytoplankton distribution; High resolution; Automated flow cytometry; Fronts; Mesoscale structure.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00601/71324/69752.pdf
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Symbolic Analysis of Plankton Swimming Trajectories: Case Study of Strobilidium sp. (Protista) Helical Walking under Various Food Conditions ArchiMer
Vandromme, Pieter; Schmitt, François G.; Souissi, Sami; Buskey, Edward J.; Strickler, J. Rudi; Wu, Cheng-han; Hwang, Jiang-shiou.
The swimming behavior of the ciliate Strobilidium sp. was recorded using cinematographic techniques. A density of 20 ciliates/ml was used under 4 experimental food conditions: 121, 625, 3025, and 15,125 cells/ml of the dinoflagellate Gymnodinium sp. In total, 100 trajectories per experiment were recorded and analyzed. We classified this ciliate’s swimming trajectories into categories we called “helix”, “non-helix”, and “break”. These swimming states were identified using automated recognition of helices, based on values of swimming trajectory angles. We performed a symbolic analysis of the succession of swimming states which enabled discrimination between food concentration experiments, and provided a more-complete characterization of the swimming...
Tipo: Text Palavras-chave: Protista; Plankton behavior; Swimming states; Symbolic dynamics; Simulation.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00070/18171/15730.pdf
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