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	Provedor de dados ArchiMer (1) Autor Atmar, Robert (1) Estes, Mary (1) Le Guyader, Soizick (1) Neill, Frederick (1) Schwab, Kellogg (1) Palavra-chave Bivalvia (1) DNA enzyme (1) Detection method (1) Enteric virus (1) Immuoessay (1) Reverse transcription PCR (1) Mais... Tipo do documento Text (1) Ano 2001 (1) País France (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Development of a reverse transcription-PCR-DNA enzyme immunoassay for detection of "Norwalk-like" viruses and hepatitis A virus in stool and shellfish ArchiMer Schwab, Kellogg; Neill, Frederick; Le Guyader, Soizick; Estes, Mary; Atmar, Robert. Outbreaks of food- and waterborne gastroenteritis are being increasingly reported throughout the world. The analysis of environmental samples by ne,ver diagnostic techniques such as reverse transcription-PCR (RT-PCR) amplification of nucleic acid has begun to identify human enteric viruses (predominantly "Norwalklike" viruses [NLVs]) as the cause of many of these outbreaks. To streamline NLV detection from environmental samples such as shellfish, we have developed an RT-PCR-oligoprobe amplification and detection method using several new procedures that enable confirmed RT-PCR amplification and product detection in 1 day. The new steps include replacing reverse transcriptase and Tag polymerase with rTth polymerase, a heat-stable enzyme that functions as... Tipo: Text Palavras-chave: Bivalvia; Immuoessay; DNA enzyme; Reverse transcription PCR; Detection method; Enteric virus. Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-1267.pdf Registros recuperados: 1 Primeira ... 1 ... Última

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