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| Magalhães-Matos,Paulo Cesar; Silva,Michele Bahia do Vale; Souza,Paulo Geovani Silva; Lima,Danillo Henrique da Silva; Morais,Rodrigo de; Farias,Diana Maria de; Alvarenga,Laís Cristina Oliveira; Gabriel,Áurea Martins; Oliveira,Wanessa Batista Lima; Góes-Cavalcante,Gustavo; Scofield,Alessandra. |
| ABSTRACT: The objective of this study was to detect helminth eggs and protozoan oocysts in samples of feces from birds of the order Passeriformes in Para State, Brazil. Fecal samples were collected individually from 403 passerine birds seized and kept in captivity in Para State. Samples were processed by the double centrifugation technique in saturated sucrose solution and the coccidial oocyst-positive samples were submitted to sporulation in potassium dichromate 2.0%. Helminth eggs and/or protozoan oocysts were observed in 43.18% (174/403) of the fecal samples examined. Coccidial oocysts were detected in 93.68% (163/174) of the positive samples, whereas helminth eggs were observed in 10.34% (18/174) of the positive samples. Oocyst sporulation occurred in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passerine birds; Endoparasites; Coccidia; Helminths; Brazilian Amazon. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202177 |
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| Magalhães-Matos,Paulo Cesar; Cunha-Santos,Rafaelle; Sousa,Paulo Geovani Silva; Sampaio-Júnior,Francisco Dantas; Barros,Flávia de Nazaré Leite; Mourão,Fábio Rodrigo Paixão; Oliveira,Wanessa Batista Lima; Gabriel,Áurea Martins; Monteiro,Silvia Gonzalez; Góes-Cavalcante,Gustavo; Scofield,Alessandra. |
| ABSTRACT: The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma evansi; PCR; Procyonidae; Eastern Amazon. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400663 |
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| Silva,Andréia Ferreira da; Damasceno,Ágatha Rossanni Alves; Prado,Welton Seabra; Caldeira,Rossela Damasceno; Sampaio-Junior,Francisco Dantas; Farias,Diana Maria de; Silva,Laís Cristina Oliveira da; Guimarães,Ricardo José de Paula Souza e; Góes-Cavalcante,Gustavo; Scofield,Alessandra. |
| ABSTRACT: This study was designed to detect L. infantum infection in dogs and to evaluate the factors associated with canine visceral leishmaniasis in the maroon communities of Menino Jesus de Petimandeua and Itaboca in the municipality of Inhangapi, Pará, Brazil. Whole blood and intact skin samples were collected from 143 dogs, and a questionnaire was applied. L. infantum DNA was detected by polymerase chain reaction (PCR) using primers RV1 and RV2. Collection sites were georeferenced to obtain a spatial distribution of the residences visited and infected dogs. L. infantum DNA was detected in 8.4% (12/143) of the skin samples and in 1.4% (2/143) of the blood samples. On the risk map, three clusters were observed in Itaboca and one was observed in Menino... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania infantum; Maroon communities; Dog; PCR; Eastern Amazon. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100501 |
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| Silva,Josileide Araújo da; Domiciano,Tarcísio Oliveira; Montão,Daniele Pina; Sousa,Paulo Geovani Silva; Ramos,Leandro Lopes; Paredes,Laura Jamille Argolo; Monteiro,Silvia Gonzalez; Rivero,Gabriela Riet Correa; Scofield,Alessandra; Bezerra Júnior,Pedro; Bezerra,Isis Abel; Cerqueira,Valíria Duarte. |
| ABSTRACT: Two outbreaks of trypanosomiasis by Trypanosoma evansi in horses in the municipality of Chaves, Marajó Island, Pará State, Brazil are reported. The first outbreak occurred in April 2011 in a farm with 147 horses; of these, 47 (31.97%) got sick, and 40 (27.21%) died. The second outbreak occurred in May 2012 and involved nine properties. Of a total of 679 horses, 209 (30.07%) got sick, and 183 (26.97%) died. The main clinical signs observed in these horses were weight loss, abdominal edema, toe dragging, and pelvic muscle atrophy. Necropsy was performed in two horses, one from each outbreak. Macroscopic alterations were not seen in the horse from the first outbreak; however, the horse from the second outbreak exhibited emaciation, pallor and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equids; Trypanosomiasis; Pará; Marajó Island. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202170 |
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| Scofield,Alessandra; Madureira,Renata Cunha; Oliveira,Carlo José Freire de; Guedes Junior,Daniel da Silva; Soares,Cleber Oliveira; Fonseca,Adivaldo Henrique da. |
| Os objetivos deste trabalho foram diagnosticar Aelurostrongylus abstrusus por meio das técnicas de histologia e impressão de tecido pulmonar de gato doméstico e realizar a caracterização morfométrica de ovos e mórulas desse nematódeo. Pela histologia, os ovos (n=100) mediram, em média, 83,4 x 65,5mim, índice morfométrico (IM) 0,8 e as mórulas (n=100) mediram, em média, 45,4 x 31,8mim, IM 0,7. Pela impressão de pulmão, os ovos (n=100) mediram, em média, 105,3 x 93,6mim, IM 0,9, e as mórulas (n=100) mediram, em média, 43,1 x 36,5mim, IM 0,8. A utilização da técnica de impressão de tecido permitiu a observação das mesmas formas parasitárias visualizadas histologicamente. Os resultados sugerem a viabilidade da utilização dessa técnica no diagnóstico pós-morte... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Verme pulmonar; Histologia; Impressão de tecido; Morfometria. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000400036 |
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| Scofield,Alessandra; Riera,Maria Dalila Forlano; Elisei,Carina; Massard,Carlos Luiz; Linardi,Pedro Marcos. |
| A ocorrência de Rhopalopsyllus lutzi lutzi (Baker) (Siphonaptera, Rhopalopsyllidae) foi assinalada em Canis familiaris (Linnaeus) de áreas rurais do município de Piraí, estado do Rio de Janeiro, Brasil. No período de junho 2001/novembro 2003, 51 sifonápteros foram capturados em oito cães procedentes de duas propriedades rurais do município de Piraí. Os exemplares coletados foram acondicionados em álcool etílico 70%, levados ao Laboratório de Parasitologia Animal da Universidade Federal Rural do Rio de Janeiro para contagem e sexagem. Os exemplares foram identificados como Rhopalopsyllus lutzi lutzi Baker, 1904 (machos=18 e fêmeas=33). R. lutzi lutzi é, pela primeira vez, assinalada em cães domésticos naturalmente infestados em áreas rurais do estado do Rio... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cães; Pulgas; Rhopalopsyllidae; Rhopalopsyllus lutzi lutz; Siphonaptera. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262005000100019 |
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