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| Secchi,Carina; Antunes,Ana Lúcia Souza; Perez,Leandro Reus Rodrigues; Cantarelli,Vlademir Vicente; d'Azevedo,Pedro Alves. |
| The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase-negative Staphylococcus; Cefoxitin; Oxacillin; MecA gene; Susceptibility diagnostic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012 |
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| Secchi,Carina; Cantarelli,Vlademir Vicente; Pereira,Fabiana de Souza; Wolf,Hilda Helena Chaer; Brodt,Teresa Cristina Zenobini; Amaro,Maria Cristina O.; Inamine,Éverton. |
| Anaerobiospirillum succiniciproducens is an anaerobic, Gram-negative, spiral shaped bacteria, which is motile by means of bipolar tuffs of flagella. This organism appears to be a rare cause of bacteremia in humans, and it usually affects patients submitted to immunosuppressive therapy. Anaerobiospirillum succiniciproducens resembles Campylobacter spp. in Gram-stained preparations, however, it is considered resistant to most antimicrobial drugs that are used to treat Campylobacter infections. We observed Gram-negative, spiral shaped bacteria in Gram-stained preparations from blood culture flasks. Growth occurred only under anaerobic incubation, and identification to the species level was achieved by PCR amplification of the 16S rRNA gene, followed by direct... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Bacteremia; Anaerobiospirillum spp.; Anaerobiospirillum succiniciproducens. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000200008 |
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| Cantarelli,Vlademir Vicente; Brodt,Teresa Cristina. Z.; Secchi,Carina; Inamine,Everton; Pereira,Fabiana de Souza; Pilger,Diogo Andre. |
| Corynebacterium species have often been considered normal skin flora or contaminants; however, in recent years they have been increasingly implicated in serious infections. Moreover, many new species have been discovered and old species renamed, especially after molecular biology techniques were introduced. Corynebacterium mucifaciens is mainly isolated from blood and from other normally-sterile body fluids; it forms slightly yellow, mucoid colonies on blood agar. We report a fatal case of bacteremia due to an atypical strain of C. mucifaciens. This strain had atypical colony morphology; analysis of the 16S rRNA gene was used to define the species. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Bacteremia; Blood infection; Corynebacterium; Corynebacterium mucifaciens. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600013 |
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| Cantarelli,Vlademir Vicente; Inamine,Everton; Brodt,Teresa Cristina Zenobin; Secchi,Carina; Pereira,Fabiana de Souza; Amaro,Maria Cristina; Batalha,Alice Aurora; Ligiero,Simone Dornellas. |
| Stringent quality control protocols must be used in order to guaranty that a particular medium is able to recover all sort of organism that may be present in clinical samples. Our aim was to evaluate an alternative protocol that would allow us to detect medium failure to yield quantitative growth of selected pathogens, and compare with the document M22-A2 from NCCLS. The detection limit of Haemophilus influenzae was significantly different depending on media source. We conclude that for some fastidious microorganisms, quantitative verification of the growth capacity of the culture medium is advised. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quality control; Culture medium; NCCLS. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500003 |
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