Sensory feedback is essential for the acquisition of complex motor behaviors, including birdsong. In zebra finches, auditory feedback is relayed to the descending motor pathway primarily through the nucleus interfacialis nidopalii (NIf). NIf projects to HVC - a premotor region essential for song, which projects to RA, a motor cortex analogue brain area that drives muscle activity required for vocalizations. NIf is also essential for ‘sleep replay’, a recapitulation of song-related neural dynamics in the motor pathway during sleep. Despite being one of the major inputs to the song control pathway, there is no known role for NIf in the production of zebra finch song. To address this, we reversibly inactivated NIf using TTX or Muscimol... |