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| Hu,T.; Shi,J.; Jiao,X.; Zhou,J.; Yin,X.. |
| Phosphatidylserine (PS) exposure occurs during the cell death program and fluorescein-labeled lactadherin permits the detection of PS exposure earlier than annexin V in suspended cell lines. Adherent cell lines were studied for this apoptosis-associated phenomenon to determine if PS probing methods are reliable because specific membrane damage may occur during harvesting. Apoptosis was induced in the human tongue squamous carcinoma cell line (Tca8113) and the adenoid cystic carcinoma cell line (ACC-2) by arsenic trioxide. Cells were harvested with a modified procedure and labeled with lactadherin and/or annexin V. PS exposure was localized by confocal microscopy and apoptosis was quantified by flow cytometry. The detachment procedure without trypsinization... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adherent cells; Annexin V; Apoptosis; Flow cytometry; Lactadherin. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900002 |
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| Wu,J.; Wang,Y.; Liu,G.; Jia,Y.; Yang,J.; Shi,J.; Dong,J.; Wei,J.; Liu,X.. |
| Alveolar epithelia play an essential role in maintaining the integrity and homeostasis of lungs, in which alveolar epithelial type II cells (AECII) are a cell type with stem cell potential for epithelial injury repair and regeneration. However, mechanisms behind the physiological and pathological roles of alveolar epithelia in human lungs remain largely unknown, partially owing to the difficulty of isolation and culture of primary human AECII cells. In the present study, we aimed to characterize alveolar epithelia generated from A549 lung adenocarcinoma cells that were cultured in an air-liquid interface (ALI) state. Morphological analysis demonstrated that A549 cells could reconstitute epithelial layers in ALI cultures as evaluated by histochemistry... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alveolar epithelium; A549 cells; Air-liquid interface; Alveolar epithelial type II cells; Alveolar epithelial type I cells. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200609 |
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