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	Provedor de dados BABT (1) BJMBR (1) Genet. Mol. Biol. (1) Autor Shi,Ying (3) Du,Yue-feng (1) Gong,Weihua (1) He,Da-lin (1) Liang,Liang (1) Liu,Cheng-Cheng (1) Long,Qing-zhi (1) Lu,Lu (1) Ren,Jingjing (1) Wang,Xin-yang (1) Wang,Yunfeng (1) Yang,Ke-Wu (1) Zeng,Jin (1) Zhai,Le (1) Mais... Palavra-chave Autophagy (1) Breast cancer (1) DNA methylation (1) DNMT3 (1) HMGB1 (1) Metallo-B-lactamase CcrA (1) MiR-129-5p (1) Plateau elution profile (1) Prostate cancer (1) Purification (1) RNA interference (1) Salting-out method (1) Taxol (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2019 (1) 2013 (1) 2012 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1 Genet. Mol. Biol. Du,Yue-feng; Liang,Liang; Shi,Ying; Long,Qing-zhi; Zeng,Jin; Wang,Xin-yang; He,Da-lin. Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Prostate cancer; DNA methylation; DNMT3; RNA interference. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024 Purification of metallo-B-lactamase CcrA from Bacteroides fragilis with salting-out method BABT Liu,Cheng-Cheng; Zhai,Le; Shi,Ying; Yang,Ke-Wu. A simple and effective salting-out method was developed for the purification of the metallo-β-lactamase CcrA from Bacteroides fragilis based on the plasmid pMSZ02, in which the crude protein secreted into growth medium was precipitated by 80% sulfate saturation of the medium and purified with Q-Sepharose to offer pure CcrA with yield of 20.1 mg per litter medium. The dependence of the amount of protein precipitation on sulfate saturation was investigated, which showed that more than 80% sulfate saturation resulted the maximum protein precipitated. The purified CcrA was evaluated by steady-state kinetics using penicillin G and cephalothin V as substrates, which showed the Km values of 68±2 and 17±2 µM and Kcat values of 63±1 and 102±3 S-1, respectively. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Metallo-B-lactamase CcrA; Plateau elution profile; Purification; Salting-out method. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500012 Upregulation of miR-129-5p increases the sensitivity to Taxol through inhibiting HMGB1-mediated cell autophagy in breast cancer MCF-7 cells BJMBR Shi,Ying; Gong,Weihua; Lu,Lu; Wang,Yunfeng; Ren,Jingjing. Although Taxol has improved the survival of cancer patients as a first-line chemotherapeutic agent, an increasing number of patients develop resistance to Taxol after prolonged treatment. The potential mechanisms of cancer cell resistance to Taxol are not completely clear. It has been reported that microRNAs (miRNAs) are involved in regulating the sensitivity of cancer cells to various chemotherapeutic agents. In this study, we aimed to explore the role of miR-129-5p in regulating the sensitivity of breast cancer cells to Taxol. Cell apoptosis and autophagy, and the sensitivity of MCF-7 cells to Taxol were assessed with a series of in vitro assays. Our results showed that the inhibition of autophagy increased the Taxol-induced apoptosis and the sensitivity... Tipo: Info:eu-repo/semantics/article Palavras-chave: MiR-129-5p; HMGB1; Autophagy; Taxol; Breast cancer. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100603 Registros recuperados: 3 Primeira ... 1 ... Última

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