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	Provedor de dados BJM (2) Autor Shimizu,Mario T. (2) Cunha,Ildenize B.S. (1) Marcucci,Maria C. (1) Miyasaka,Natália R.S. (1) Sawaya,Alexandra C. H. F. (1) Souza,Kirllian S. (1) Unterkircher,Carmelinda S. (1) Mais... Palavra-chave Antigen (1) Bioauthography (1) Candida (1) Candidiasis (1) Catalase (1) Diagnosis (1) Extraction methods (1) High performance liquid chromatography (1) Minimal bactericidal concentration (1) Propolis (1) Thin layer chromatography (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2008 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria BJM Sawaya,Alexandra C. H. F.; Souza,Kirllian S.; Marcucci,Maria C.; Cunha,Ildenize B.S.; Shimizu,Mario T.. Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Thin layer chromatography; Bioauthography; High performance liquid chromatography; Extraction methods; Minimal bactericidal concentration. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100017 Catalase activity of different Candida species after exposition to specific antiserum BJM Miyasaka,Natália R.S.; Unterkircher,Carmelinda S.; Shimizu,Mario T.. Antisera were developed in rabbits after challenge with intracellular antigens of Candida albicans, C. tropicalis and C. parapsilosis. Microorganism catalase has been correlated with virulence, resistance to drugs and immunogenicity. The intracellular catalase is consistently present in strains of Candida and in this paper, the enzyme activity was analysed by PAGE after exposition to antisera. The catalases of C. albicans, C. parapsilosis and C. tropicalis were immunogenic and differed in their binding to specific antibodies raised in rabbits. Tests of cross-reactivity between different Candida species showed that when antiserum from C. albicans immunized rabbit was incubated with intracellular extracts of these three Candida species, the catalases... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidiasis; Catalase; Candida; Antigen; Diagnosis. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100009 Registros recuperados: 2 Primeira ... 1 ... Última

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