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	Provedor de dados Biological Sciences (1) Autor Dolabella, Silvio Santana (1) Fernandes, Marcelo Ferreira (1) Fernandes, Roberta Pereira Miranda (1) Rodrigues, Isabela da Silva Vasconcelos (1) Silva, Carolina Goes Silva e (1) Silva, Rafael Salomão da (1) Mais... Palavra-chave 2.08.00.00-2 (1) 2.08.05.00-4 alkali–thermostable enzyme (1) Bacillus spp. Enzimologia (1) Biochemical characterization (1) Soil bacteria (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2019 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching Biological Sciences Rodrigues, Isabela da Silva Vasconcelos; Silva, Carolina Goes Silva e; Silva, Rafael Salomão da; Dolabella, Silvio Santana; Fernandes, Marcelo Ferreira; Fernandes, Roberta Pereira Miranda. In this study, two hundred fifty-seven bacterial isolates from a suppressive soil library were screened to study their secretion of alkali-thermostable xylanases for potential use in cellulose pulp biobleaching. Xylanase activity was evaluated in solid and liquid media using xylan as the carbon source. Isolates were initially evaluated for the degradation of xylan in solid media by the congo red test. Selected strains were evaluated in liquid media for enzymatic activity and determination of total protein concentration using a crude protein extract (CPE). An isolate identified as Bacillus species TC-DT13 produced the highest amount of xylanase (1808 U mL-1). The isolate was active and stable at 70°C and pH 9.0, conditions which are necessary for the paper... Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.08.00.00-2; 2.08.05.00-4 alkali–thermostable enzyme; Soil bacteria; Biochemical characterization; Bacillus spp. Enzimologia. Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101 Registros recuperados: 1 Primeira ... 1 ... Última

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