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Induction and regeneration of somatic embryos of sugarcane RB996961 Sci. Agrar.Paran. / SAP
Almeida, Rodrigo de Oliveira; Silva, Sérgio Delmar dos Anjos e; Vargas, Daiane Peixoto; Dutra, Leonardo Ferreira.
Sugarcane is a crop widely planted in Brazil because of the economic importance of its products, like sugar and ethanol. Thus, the crop is often inserted in breeding programs. Therefor, the aim of this study was to evaluate the morphogenic response to somatic embryogenesis and somatic embryos regeneration for genotype RB996961. Transversal segments of young leave rolls with 2-3 mm size from plants with 6 to 9 months old were subcultured on solid MS medium supplemented with three concentrations of 2.4-dichlorophenoxyacetic acid (2.4-D) for embryogenic callus induction. Better results were obtained using 13.5 or 31.5 µM L-1 of 2.4-D in 45 or 60 days of incubation on induction medium, one to three subculture and 15 to 45 days of incubation on regeneration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum spp.; Cultura de tecido vegetal; Embriogênese somática.
Ano: 2015 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9081
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Induction of somatic embryogenesis and plant regeneration of sugarcane cv. RB925345 from somatic embryos Sci. Agrar.Paran. / SAP
Almeida, Rodrigo de Oliveira; Silva, Sérgio Delmar dos Anjos e; Dutra, Leonardo Ferreira.
The aim of this study was to establish a protocol for induction of somatic embryogenesis and regeneration of somatic embryos of sugarcane (cultivar RB925345). Best results was obtained using the concentration of 13.5 µM L-1 2,4-dichlorophenoxyacetic acid, 45 days of incubation in induction medium, one subculture and 15 days of incubation in regeneration medium, providing 47.5% of embryogenic calli and 38.9% of plants regenerated. Adjust are necessary in this protocol to increase the rate of embryogenic calli production and regeneration of somatic embryos.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum spp.; 2 4-dichlorophenoxyacetic acid; Plant tissue culture; Somatic embryogenesis.
Ano: 2018 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14165
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