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Droste,Annette; Silva,Anelise Machado da; Souza,Iliane Freitas de; Wiebke-Strohm,Beatriz; Bücker-Neto,Lauro; Bencke,Marta; Sauner,Márcia Viviane; Bodanese-Zanettini,Maria Helena. |
The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Brazilian cultivars; Embryogenic potential; Genetic engineering; Soybean transformation. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700012 |
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