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The role of matrix effects on the quantification of abscisic acid and its metabolites in the leaves of Bauhinia variegata L. using liquid chromatography combined with tandem mass spectrometry Braz. J. Plant Physiol.
Silva,Carolina M. Santiago da; Habermann,Gustavo; Marchi,Mary R. R.; Zocolo,Guilherme J..
Phytohormone analysis using liquid chromatography combined with tandem mass spectrometry is a very important tool for studies involving plant metabolism; however, this analysis can be affected by matrix composition. Although it is necessary to understand this effect to produce reliable results, it has been widely neglected in analyses of plant hormones. Leaf extracts from Bauhinia variegata were obtained by solvent extraction followed by solid-phase cleanup. The extract was analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) in negative ionization mode, using the multiple reaction monitoring mode with two or three transitions to enhance analytical quality control. Although deuterated standards were used as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ABA-β-D-glucosyl ester; ABA daily variations; Dihydrophaseic acid; LC-ESI-MS/MS; Phaseic acid.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000300009
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