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An efficient system for in vitro propagation of Bouchea fluminensis (Vell.) Mold. (Verbenaceae) Acta Botanica
Resende,Cristiano Ferrara de; Braga,Virgínia Fernandes; Silva,Cristiane Jovelina da; Pereira,Paula da Fonseca; Ribeiro,Cleberson; Salimena,Fátima Regina Gonçalves; Peixoto,Paulo Henrique Pereira.
This study aimed to establish and propagate in vitro plants of Bouchea fluminensis, a medicinal species known in Brazil as gervão-falso ("false verbena"), evaluating the influences of different growth regulators on in vitro multiplication and rooting stages, as well as examining ex vitro acclimatization of rooted plants. Explants were established on Murashige and Skoog medium at half strength of salts and vitamins without growth regulators. For multiplication, the explants were subjected to combinations of 6-benzyladenine (BA; 0, 2.5, 5.0 and 7.5 µM) and α-naphthalene-acetic acid (NAA; 0, 0.2, 0.4 and 0.6 µM). The medium found to induce the greatest number of shoot was that containing 5 µM of BA (NAA-free). For rooting, we evaluated three auxins (NAA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Biodiversity conservation; Espinhaço Mountain Range.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200005
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Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins Acta Botanica
Cardoso,Amanda Ávila; Borges,Eduardo Euclydes de Lima e; Souza,Genaina Aparecida de; Silva,Cristiane Jovelina da; Pires,Raquel Maria de Oliveira; Dias,Denise Cunha Fernandes dos Santos.
ABSTRACTStudies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaporins; Dithiothreitol; Forest species; Mercury; Seed.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300285
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