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Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis BABT
Chaud,Luciana Cristina Silveira; Silva,Débora Danielle Virgínio da; Mattos,Rafael Taino de; Felipe,Maria das Graças de Almeida.
Oat hull hemicellulosic hydrolysate obtained by diluted acid hydrolysis was employed as fermentation medium for Pichia stipitis cultivation. A comparison between the use of treated hydrolysate with 1% activated charcoal to reduce the toxic compounds generated during the hydrolysis process and untreated hydrolysate as a control was conducted. In the cultures using treated hydrolysate the total consumption of glucose, low xylose consumption and ethanol and glycerol formation were observed. The medium formulated with untreated hydrolysate showed morphological cell modifications with consequently cell death, no ethanol formation and formation of glycerol as byproduct of fermentative process, probably as a response to stressful conditions to yeast due to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pichia stipitis; Hemicellulosic hydrolysate; Oat hull; Ethanol; Activated charcoal.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500017
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Improvement of biotechnological xylitol production by glucose during cultive of Candida guilliermondii in sugarcane bagasse hydrolysate BABT
Silva,Débora Danielle Virgínio da; Mancilha,Ismael Maciel de; Silva,Silvio Silvério da; Felipe,Maria das Graças de Almeida.
The effect of glucose on xylose-to-xylitol bioconversion by Candida guilliermondii was examined by adding it to sugarcane bagasse hydrolysate medium to obtain different glucose:xylose ratios (1:25, 1:12, 1:5 and 1:2.5). Under experimental conditions, increasing glucose:xylose ratio improved the assimilation of the xylose present in the hydrolysate by yeast, resulting in biomass increase, and in the formation of xylitol and glycerol/ethanol by-products. Maximum values of xylitol yield (0.59 g g-1) and volumetric productivity (0.53 g l-1.h-1) were obtained with glucose:xylose ratio of 1:5, resulting in the higher conversion efficiency (64.3%).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida guilliermondii; Glucose; Sugarcane bagasse hydrolysate; Xylitol; Xylose.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200005
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