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| Andrade,S.A.; Carrijo-Carvalho,L.C.; Peceguini,L.A.M.; Wlian,L.; Sato,A.C.; Luchiari,C.; Silva,E.D.; Maffei,F.H.A.; Chudzinski-Tavassi,A.M.. |
| Lopap, found in the bristles of Lonomia obliqua caterpillar, is the first exogenous prothrombin activator that shows serine protease-like activity, independent of prothrombinase components and unique lipocalin reported to interfere with hemostasis mechanisms. To assess the action of an exogenous prothrombin activator reversing the anticoagulant and antihemostatic effect induced by low molecular weight heparin (LMWH), male New Zealand rabbits (N = 20, weighing 3.8-4.0 kg) allocated to 4 groups were anticoagulated with 1800 IU/kg LMWH (iv) over 2 min, followed by iv administration of saline (SG) or recombinant Lopap (rLopap) at 1 µg/kg (LG1) or 10 µg/kg (LG10), 10 min after the injection of LMWH, in a blind manner. Control animals (CG) were treated only with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prothrombin activation; Procoagulant; Exogenous hemostatic factor; RLopap; Bleeding; Anticoagulant. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000007 |
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| Nascimento,D.V.; Lemes,E.M.B.; Queiroz,J.L.S.; Silva Jr.,J.G.; Nascimento,H.J.; Silva,E.D.; Hirata Jr.,R.; Dias,A.A.S.O.; Santos,C.S.; Pereira,G.M.B.; Mattos-Guaraldi,A.L.; Armoa,G.R.G.. |
| The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently available. In the present study, the genomic DNA of the Corynebacterium diphtheriae Park Williams 8 (PW8) vaccine strain was used as a template for PCR amplification of the dtb gene. After amplification, the dtb gene was cloned and expressed in competent Escherichia coli M15™ cells using the expression vector pQE-30™. The lysate obtained from transformed E. coli cells containing the rDTB PW8 was clarified by centrifugation and purified by affinity chromatography. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragment B; Diphtheria toxin; Diphtheria; Dtb gene; E. coli gene expression; Immobilized metal affinity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500007 |
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